C57BL/6JCya-Ogtem1flox/Cya
Common Name:
Ogt-flox
Product ID:
S-CKO-00668
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ogt-flox
Strain ID
CKOCMP-108155-Ogt-B6J-VA
Gene Name
Product ID
S-CKO-00668
Gene Alias
1110038P24Rik; 4831420N21Rik; Ogtl
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ogtem1flox/Cya mice (Catalog S-CKO-00668) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044475
NCBI RefSeq
NM_139144
Target Region
Exon 2~3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Ogt, short for O-GlcNAc transferase, is a key protein in O-linked N-acetylglucosamine (O-GlcNAc) post-translational modification. It catalyzes O-GlcNAcylation by attaching GlcNAc from glycosyl donors to protein Ser/Thr residues, regulating multiple biological processes such as signal transduction, cell cycle, metabolism, and energy homeostasis [2,3,7]. It is involved in pathways like NF-κB signaling [1], and its function is crucial for maintaining cell homeostasis in various cell types [5]. Genetic models, especially KO/CKO mouse models, are valuable tools to study its functions.
Ogt deficiency in mice leads to inflammatory activation of astrocytes both in vivo and in vitro, impairing cognitive function. GlcNAc supplementation to restore O-GlcNAcylation can inhibit astrocyte activation, inflammation, and improve cognitive function. Mechanistically, Ogt interacts with NF-κB p65 and catalyzes its O-GlcNAcylation, while Ogt deficiency promotes Gsk3β binding, activating the NF-κB signaling pathway [1]. In mouse embryonic stem cells, Ogt deficiency blocks cell proliferation due to mitochondrial dysfunction secondary to mTOR hyperactivation. Ogt normally maintains low mTOR activity and mitochondrial fitness by suppressing proteasome activity [4]. In clear cell renal cell carcinoma, Ogt promotes tumor progression by increasing the protein level of HIF-2α through repressing its ubiquitin-proteasome system-mediated degradation [6].
In conclusion, Ogt is essential for many biological processes. Through model-based research, especially KO/CKO mouse models, we've learned that Ogt plays a critical role in processes related to astrocyte activation and cognitive function, cell viability, and cancer progression. These findings contribute to understanding diseases such as neurodegenerative disorders and cancers, providing potential therapeutic targets for treatment.
References:
1. Dong, Xiaoxue, Shu, Liqi, Zhang, Jinyu, Shu, Qiang, Li, Xuekun. 2023. Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway. In Journal of neuroinflammation, 20, 146. doi:10.1186/s12974-023-02824-8. https://pubmed.ncbi.nlm.nih.gov/37349834/
2. Zhang, Ning, Jiang, Hongli, Zhang, Kexin, Ye, Feng, Qu, Wei. 2022. OGT as potential novel target: Structure, function and inhibitors. In Chemico-biological interactions, 357, 109886. doi:10.1016/j.cbi.2022.109886. https://pubmed.ncbi.nlm.nih.gov/35288161/
3. Liu, Xin, Wang, Jing, Xiang, Yaoxian, Yan, Dong, Tong, Yingying. 2024. The roles of OGT and its mechanisms in cancer. In Cell & bioscience, 14, 121. doi:10.1186/s13578-024-01301-w. https://pubmed.ncbi.nlm.nih.gov/39285476/
4. Li, Xiang, Yue, Xiaojing, Sepulveda, Hugo, A Myers, Samuel, Rao, Anjana. 2023. OGT controls mammalian cell viability by regulating the proteasome/mTOR/ mitochondrial axis. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2218332120. doi:10.1073/pnas.2218332120. https://pubmed.ncbi.nlm.nih.gov/36626549/
5. Potter, Sarah C, Gibbs, Bettine E, Hammel, Forrest A, Haggarty, Stephen J, Walker, Suzanne. 2024. Dissecting OGT's TPR domain to identify determinants of cellular function. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2401729121. doi:10.1073/pnas.2401729121. https://pubmed.ncbi.nlm.nih.gov/38768345/
6. Yang, Zhou, Wei, Xiyi, Ji, Chengjian, Song, Ninghong, Qin, Chao. 2023. OGT/HIF-2α axis promotes the progression of clear cell renal cell carcinoma and regulates its sensitivity to ferroptosis. In iScience, 26, 108148. doi:10.1016/j.isci.2023.108148. https://pubmed.ncbi.nlm.nih.gov/37915611/
7. He, Xue-Fen, Hu, Xiaoli, Wen, Gao-Jing, Wang, Zhiwei, Lin, Wen-Jing. 2023. O-GlcNAcylation in cancer development and immunotherapy. In Cancer letters, 566, 216258. doi:10.1016/j.canlet.2023.216258. https://pubmed.ncbi.nlm.nih.gov/37279852/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen