C57BL/6JCya-E2f8em1flox/Cya
Common Name:
E2f8-flox
Product ID:
S-CKO-00727
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
E2f8-flox
Strain ID
CKOCMP-108961-E2f8-B6J-VA
Gene Name
Product ID
S-CKO-00727
Gene Alias
4432406C08Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-E2f8em1flox/Cya mice (Catalog S-CKO-00727) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000058745
NCBI RefSeq
NM_001013368
Target Region
Exon 3~4
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
E2F8, a member of the E2F family, is a multifaceted transcription factor. It has a duplicated DNA-binding domain, distinguishing it from E2F1-6, and controls gene expression in a dimerization-partner-independent manner. E2F8 is crucial for angiogenesis, lymphangiogenesis, and embryonic development, and is involved in pathways like cell-cycle regulation, such as the G1-to-S-phase transition [1].
E2F8 shows diverse roles in different diseases. In hepatocellular carcinoma (HCC), it promotes cancer occurrence and development via the E2F1/Cyclin D1 signaling pathway or by transcriptionally suppressing CDK1 [1]. In cervical and ovarian cancers, E2F8 is associated with cancer progression, regulating cell proliferation, invasion, and epithelial-mesenchymal transition (EMT) [2,5]. In breast cancer, E2F8 contributes to homologous recombination repair and chemoresistance through the E2F8-CENPL pathway [4]. In contrast, in the human placenta, it may suppress the invasiveness of extravillous trophoblasts, different from its role in the murine placenta [3].
In conclusion, E2F8 is essential for multiple biological processes and embryonic development. Its dysregulation is closely associated with various cancers, making it a potential therapeutic target. Research on E2F8, especially through gene-knockout or conditional-knockout models in mice, helps in understanding its role in disease mechanisms, offering insights for developing new treatment strategies for cancer and other related diseases.
References:
1. Lv, Yi, Xiao, Jia, Liu, Jing, Xing, Feiyue. 2017. E2F8 is a Potential Therapeutic Target for Hepatocellular Carcinoma. In Journal of Cancer, 8, 1205-1213. doi:10.7150/jca.18255. https://pubmed.ncbi.nlm.nih.gov/28607595/
2. Kim, Lee Kyung, Park, Sun-Ae, Eoh, Kyung Jin, Kim, Young Tae, Kim, Hee Jung. 2020. E2F8 regulates the proliferation and invasion through epithelial-mesenchymal transition in cervical cancer. In International journal of biological sciences, 16, 320-329. doi:10.7150/ijbs.37686. https://pubmed.ncbi.nlm.nih.gov/31929759/
3. Mizuno, Masako, Miki, Rika, Moriyama, Yoshinori, Kikkawa, Fumitaka, Kotani, Tomomi. 2018. The role of E2F8 in the human placenta. In Molecular medicine reports, 19, 293-301. doi:10.3892/mmr.2018.9617. https://pubmed.ncbi.nlm.nih.gov/30387815/
4. Wang, Shan, Xia, Yuhong, Sun, Yu, Zhang, Zhongbo, Zhao, Chenghai. 2024. E2F8-CENPL pathway contributes to homologous recombination repair and chemoresistance in breast cancer. In Cellular signalling, 118, 111151. doi:10.1016/j.cellsig.2024.111151. https://pubmed.ncbi.nlm.nih.gov/38522807/
5. Eoh, Kyung Jin, Kim, Hee Jung, Lee, Jong Woo, Kim, Young Tae, Koo, Peter J. 2020. E2F8 Induces Cell Proliferation and Invasion through the Epithelial-Mesenchymal Transition and Notch Signaling Pathways in Ovarian Cancer. In International journal of molecular sciences, 21, . doi:10.3390/ijms21165813. https://pubmed.ncbi.nlm.nih.gov/32823614/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen