Pkn2, also known as Protein kinase N2, PRK2 or PKNγ, is a PKC-related serine/threonine-protein kinase. It is involved in multiple biological processes such as cell cycle progression, cell migration, adhesion, cytoskeleton organization, and transcription activation signaling processes [5,7]. Pkn2 is associated with pathways like DUSP6-Erk1/2, Hippo, and AKT-mTOR, highlighting its significance in cellular regulation [1,2,8].

In colon cancer, Pkn2 inhibits M2 phenotype polarization of tumor-associated macrophages via the DUSP6-Erk1/2 pathway, suppressing tumor growth [1]. In pancreatic stellate cells, loss of Pkn2 disrupts myofibroblast differentiation, promoting pancreatic tumor invasion [3]. In cardiomyocytes, Pkn2 knockout leads to prenatal 'congenital' cardiomyopathy and defective angiotensin II stress responses [4]. In fibroblasts, depletion of Pkn2 by Cre-recombinase affects cell motility and N-cadherin expression, impairing cell aggregation and spheroid formation in suspension culture [6].

In summary, Pkn2 plays crucial roles in various biological processes and diseases. Gene knockout mouse models, such as those in colon cancer, pancreas, heart, and fibroblasts, have revealed its importance in tumor growth regulation, cell-cell interactions, and cardiac development and stress responses. These studies provide insights into potential therapeutic strategies targeting Pkn2 in relevant diseases.