C57BL/6JCya-Dutem1flox/Cya
Common Name:
Dut-flox
Product ID:
S-CKO-00824
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dut-flox
Strain ID
CKOCMP-110074-Dut-B6J-VA
Gene Name
Product ID
S-CKO-00824
Gene Alias
5031412I06Rik; 5133400F09Rik; D2Bwg0749e; Dutp; dUTPase
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dutem1flox/Cya mice (Catalog S-CKO-00824) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000051605
NCBI RefSeq
NM_023595
Target Region
Exon 4~6
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
DUT, encoding deoxyuridine triphosphatase, is a key enzyme in nucleotide metabolism. It plays a crucial role in preventing uracil misincorporation during DNA replication, maintaining genomic stability. There are multiple isoforms in human cells, including nuclear (DUT-N), mitochondrial (DUT-M), DUT-3 without a localization signal, and DUT-4 with the same nuclear localization signal as DUT-N [2]. DUT-M is also involved in RLR-VISA-mediated antiviral signaling and mitochondrial metabolism [3].
In multiple myeloma, DUT knockdown by RNAi minimized bortezomib resistance, decelerated cell growth, and augmented apoptosis. In contrast, DUT overexpression enhanced resistance. DUT inhibition attenuated mitochondrial modulation and restricted MM progression [1]. In hepatocellular carcinoma, DUT knockout suppressed cell proliferation through cell cycle arrest and induced DNA damage. Targeting DUT's dUTPase activity with TAS-114 could suppress HCC growth and enhance sensitivity to Sorafenib [4].
In conclusion, DUT is essential for nucleotide metabolism and genomic stability. Its manipulation shows potential in treating multiple myeloma and hepatocellular carcinoma, highlighting the importance of DUT-related gene knockout models in understanding disease mechanisms and developing therapeutic strategies.
References:
1. Wang, Yafei, Gao, Shuang, Chen, Lin, Cao, Zeng, Li, Qian. . DUT enhances drug resistance to proteasome inhibitors via promoting mitochondrial function in multiple myeloma. In Carcinogenesis, 43, 1030-1038. doi:10.1093/carcin/bgac071. https://pubmed.ncbi.nlm.nih.gov/36426924/
2. Rácz, Gergely Attila, Nagy, Nikolett, Várady, György, Apáti, Ágota, Vértessy, Beáta G. 2023. Discovery of two new isoforms of the human DUT gene. In Scientific reports, 13, 7760. doi:10.1038/s41598-023-32970-1. https://pubmed.ncbi.nlm.nih.gov/37173337/
3. Weng, Guang-Xiu, Ling, Ting, Hou, Wen, Wang, Dan-Dan, Xu, Liang-Guo. 2021. Mitochondrial DUT-M potentiates RLR-mediated antiviral signaling by enhancing VISA and TRAF2 association. In Molecular immunology, 132, 117-125. doi:10.1016/j.molimm.2021.01.023. https://pubmed.ncbi.nlm.nih.gov/33582548/
4. Xu, Mingjing, Liu, Yue, Wan, Ho Lee, Ng, Kelvin K-C, Wong, Nathalie. 2022. Overexpression of nucleotide metabolic enzyme DUT in hepatocellular carcinoma potentiates a therapeutic opportunity through targeting its dUTPase activity. In Cancer letters, 548, 215898. doi:10.1016/j.canlet.2022.215898. https://pubmed.ncbi.nlm.nih.gov/36075487/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen