C57BL/6JCya-Nop2em1flox/Cya
Common Name:
Nop2-flox
Product ID:
S-CKO-00831
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nop2-flox
Strain ID
CKOCMP-110109-Nop2-B6J-VA
Gene Name
Product ID
S-CKO-00831
Gene Alias
120kDa; A530002O17; Nol1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nop2em1flox/Cya mice (Catalog S-CKO-00831) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044200
NCBI RefSeq
NM_138747
Target Region
Exon 3~5
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Nop2, also known as NSUN1, is an S-adenosyl-L-methionine-dependent methyltransferase belonging to the NOL1/NOP2/SUN domain (NSUN) family. It is crucial for catalyzing the post-transcriptional modification of RNA through 5-methylcytosine (m5C) [2,4]. This m5C modification is involved in multiple biological processes, including ribosome biogenesis, cell cycle regulation, and cell proliferation [2,5].
In hepatocellular carcinoma (HCC), Nop2 knockout in combination with sorafenib enhanced sorafenib sensitivity and inhibited tumor growth. Mechanistically, NOP2 regulates c-Myc expression via m5C-modification to promote glycolysis, and m5C methylation of c-Myc mRNA leads to its degradation in an EIF3A-dependent manner. Also, NOP2 increases the expression of glycolytic genes [1]. In clear cell renal cell carcinoma (ccRCC), loss-of-function assays showed that NOP2 affects cell proliferation, migration, and invasion. It stimulates m5C modification of APOL1 mRNA, which is then stabilized by YBX1, and this affects ccRCC progression via the PI3K-Akt signaling pathway [3].
In conclusion, Nop2 plays significant roles in regulating biological processes through m5C-mediated RNA modification. Gene knockout models, especially in the context of cancers like HCC and ccRCC, have revealed its importance in tumor progression, metabolism reprogramming, and potential as a therapeutic target. These findings contribute to a better understanding of disease mechanisms and may offer new strategies for treatment.
References:
1. Zhang, Hao, Zhai, Xiangyu, Liu, Yanfeng, Jin, Bin, Guo, Deliang. 2023. NOP2-mediated m5C Modification of c-Myc in an EIF3A-Dependent Manner to Reprogram Glucose Metabolism and Promote Hepatocellular Carcinoma Progression. In Research (Washington, D.C.), 6, 0184. doi:10.34133/research.0184. https://pubmed.ncbi.nlm.nih.gov/37398932/
2. Liao, Han, Gaur, Anushri, McConie, Hunter, Breton, Ghislain, Denicourt, Catherine. . Human NOP2/NSUN1 regulates ribosome biogenesis through non-catalytic complex formation with box C/D snoRNPs. In Nucleic acids research, 50, 10695-10716. doi:10.1093/nar/gkac817. https://pubmed.ncbi.nlm.nih.gov/36161484/
3. Tian, Junjie, Gao, Jianguo, Cheng, Cheng, Fu, Guanghou, Jin, Baiye. 2024. NOP2-mediated 5-methylcytosine modification of APOL1 messenger RNA activates PI3K-Akt and facilitates clear cell renal cell carcinoma progression. In International journal of biological sciences, 20, 4853-4871. doi:10.7150/ijbs.97503. https://pubmed.ncbi.nlm.nih.gov/39309431/
4. Yang, Ying, Fan, Hongzhao, Liu, Hongyang, Liu, Hongchun, Wan, Junhu. 2024. NOP2 facilitates EZH2-mediated epithelial-mesenchymal transition by enhancing EZH2 mRNA stability via m5C methylation in lung cancer progression. In Cell death & disease, 15, 506. doi:10.1038/s41419-024-06899-w. https://pubmed.ncbi.nlm.nih.gov/39013911/
5. Feng, Jingyu, Zhang, Jing, Li, Yang, Mo, Panyan, Lin, Lizhu. . Upregulated expression of NOP2 predicts worse prognosis of gastric adenocarcinoma by promoting tumor growth. In Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association, 28, 369-377. doi:10.4103/sjg.sjg_573_21. https://pubmed.ncbi.nlm.nih.gov/35381832/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen