C57BL/6JCya-Fgbem1flox/Cya
Common Name:
Fgb-flox
Product ID:
S-CKO-00834
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fgb-flox
Strain ID
CKOCMP-110135-Fgb-B6J-VA
Gene Name
Product ID
S-CKO-00834
Gene Alias
2510049G14Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fgbem1flox/Cya mice (Catalog S-CKO-00834) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000048246
NCBI RefSeq
NM_181849
Target Region
Exon 2~3
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Fgb, which encodes the Bβ chain of fibrinogen, is a crucial gene. Fibrinogen, a hexameric plasmatic glycoprotein composed of pairs of three chains (Aα, Bβ, and γ), plays an essential role in hemostasis. The conversion of fibrinogen to insoluble polymer fibrin provides structural stability, strength, and adhesive surfaces for blood clots. It also has antithrombotic properties after fibrin clot formation. Besides hemostasis and thrombosis, fibrinogen and fibrin are involved in multiple biological processes such as fibrinolysis, matrix physiology, wound healing, inflammation, infection, cell interaction, angiogenesis, tumour growth, and metastasis [1].
Mutations in Fgb can lead to congenital fibrinogen deficiencies, which are rare bleeding disorders with extensive genetic heterogeneity. These mutations can cause variable clinical manifestations, ranging from asymptomatic conditions to life-threatening bleeds or thromboembolic events. For example, a novel homozygous missense mutation in FGB exon 5, p.Cys241Tyr, named "Fibrinogen Krakow V", was found in a male patient with congenital afibrinogenemia who presented with recurrent intracranial hemorrhages [6]. In addition, Fgb expression is abnormally regulated in several cancers. In renal cell carcinoma (RCC), SIRT1 downregulates FGB expression to inhibit tumorigenesis by destabilizing STAT3 [2]. In breast cancer, FGB is more highly expressed, and miR-877-5p can inhibit epithelial-mesenchymal transformation, cell proliferation, and invasion of breast cancer cells via downregulating FGB [4]. In lung adenocarcinoma (LUAD), circ_16601 promotes LUAD progression through the miR-5580-5p/FGB axis by facilitating Hippo pathway signaling [5]. Also, FGB and FGG derived from plasma exosomes might be potential biomarkers to distinguish benign from malignant pulmonary nodules [3].
In conclusion, Fgb is essential for the normal function of fibrinogen in hemostasis and a wide range of biological processes. Its abnormal regulation is associated with various bleeding and thrombotic disorders, as well as cancers. The study of Fgb-related mutations and its expression regulation in different disease models helps to understand the underlying molecular mechanisms, providing potential targets for disease diagnosis, prognosis, and treatment.
References:
1. Simurda, Tomas, Brunclikova, Monika, Asselta, Rosanna, Stasko, Jan, Kubisz, Peter. 2020. Genetic Variants in the FGB and FGG Genes Mapping in the Beta and Gamma Nodules of the Fibrinogen Molecule in Congenital Quantitative Fibrinogen Disorders Associated with a Thrombotic Phenotype. In International journal of molecular sciences, 21, . doi:10.3390/ijms21134616. https://pubmed.ncbi.nlm.nih.gov/32610551/
2. Chen, Yanbing, Zhu, Ying, Sheng, Yanling, Zhang, Shouhua, Xiang, Tianxin. 2019. SIRT1 downregulated FGB expression to inhibit RCC tumorigenesis by destabilizing STAT3. In Experimental cell research, 382, 111466. doi:10.1016/j.yexcr.2019.06.011. https://pubmed.ncbi.nlm.nih.gov/31201813/
3. Kuang, Muyu, Peng, Yizhou, Tao, Xiaoting, Sun, Yihua, Zhang, Huibiao. 2019. FGB and FGG derived from plasma exosomes as potential biomarkers to distinguish benign from malignant pulmonary nodules. In Clinical and experimental medicine, 19, 557-564. doi:10.1007/s10238-019-00581-8. https://pubmed.ncbi.nlm.nih.gov/31576477/
4. Liu, Haixia, Xiang, Lili, Mei, Yu. 2022. miR-877-5p Inhibits Epithelial Mesenchymal Transformation of Breast Cancer Cells by Targeting FGB. In Disease markers, 2022, 4882375. doi:10.1155/2022/4882375. https://pubmed.ncbi.nlm.nih.gov/36438895/
5. Zhou, Jie, Li, Peiwei, Zhao, Xiaogang, Li, Yuliang, Tian, Zhongxian. 2023. Circ_16601 facilitates Hippo pathway signaling via the miR-5580-5p/FGB axis to promote my-CAF recruitment in the TME and LUAD progression. In Respiratory research, 24, 276. doi:10.1186/s12931-023-02566-4. https://pubmed.ncbi.nlm.nih.gov/37953225/
6. Zdziarska, Joanna, Wypasek, Ewa, Iwaniec, Teresa, Neerman-Arbez, Marguerite, Undas, Anetta. 2020. Afibrinogenemia caused by a novel homozygous missense mutation, FGB p.Cys241Tyr, in a male patient with recurrent intracranial bleeding: case report and review of literature. In Haemophilia : the official journal of the World Federation of Hemophilia, 27, 26-32. doi:10.1111/hae.14211. https://pubmed.ncbi.nlm.nih.gov/33245842/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen