C57BL/6JCya-Akr7a5em1flox/Cya
Common Name:
Akr7a5-flox
Product ID:
S-CKO-00843
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Akr7a5-flox
Strain ID
CKOCMP-110198-Akr7a5-B6J-VA
Gene Name
Product ID
S-CKO-00843
Gene Alias
0610025K21Rik; Afar; Afar1; Akr7a2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Akr7a5em1flox/Cya mice (Catalog S-CKO-00843) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000073787
NCBI RefSeq
NM_025337
Target Region
Exon 2~3
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Akr7a5, an enzyme belonging to the Aldo-keto reductase-7A (AKR7A) subfamily of the AKR superfamily, plays a crucial role in aldehyde and ketone detoxification by reducing them to corresponding alcohols [4]. It is involved in protecting cells from oxidative stress and reactive aldehyde toxicity, with potential implications in multiple biological processes and diseases such as neurodegenerative diseases and cancer [4]. Genetic models, like KO mouse models, are valuable for studying its functions.
In AKR7A5-knockout (KO) mice, single ethanol binge led to more severe inflammatory response, oxidative stress, apoptosis of endogenous pathways, abnormal lipids metabolism and disordered coagulation in the liver compared to wild-type mice given alcohol [1]. This indicates that Akr7a5 deficiency exacerbates acute alcohol-induced liver injury. In vitro studies with transgenic mammalian cell lines expressing Akr7a5 showed that it protects cells from the cytotoxicity of H₂O₂, menadione, and lipid peroxidation-derived aldehydes, can lower cellular ROS levels, and maintain cellular glutathione homeostasis [2]. Also, in V79 cells, Akr7a5 expression conferred increased resistance to 4-hydroxynonenal (4-HNE) cytotoxicity, decreased the mutation rate in the presence of 4-HNE, and attenuated 4-HNE-induced caspase-3 activity, suggesting its role in protecting against 4-HNE-mediated apoptosis [3].
In conclusion, Akr7a5 is essential for protecting cells from oxidative stress and reactive aldehyde-induced damage. The KO mouse models have demonstrated its significance in preventing acute liver injury caused by alcohol, highlighting its potential importance in liver-related disease areas.
References:
1. Shi, Hui, Xu, Wenda, Liu, Qingling, Dong, Silin, Zhao, Zhenjun. . AKR7A5 knockout promote acute liver injury by inducing inflammatory response, oxidative stress and apoptosis in mice. In Journal of cellular and molecular medicine, 28, e70129. doi:10.1111/jcmm.70129. https://pubmed.ncbi.nlm.nih.gov/39365156/
2. Li, Dan, Ellis, Elizabeth M. 2014. Aldo-keto reductase 7A5 (AKR7A5) attenuates oxidative stress and reactive aldehyde toxicity in V79-4 cells. In Toxicology in vitro : an international journal published in association with BIBRA, 28, 707-14. doi:10.1016/j.tiv.2014.02.010. https://pubmed.ncbi.nlm.nih.gov/24590062/
3. Li, Dan, Hinshelwood, Alison, Gardner, Rachel, McGarvie, Gail, Ellis, Elizabeth M. 2006. Mouse aldo-keto reductase AKR7A5 protects V79 cells against 4-hydroxynonenal-induced apoptosis. In Toxicology, 226, 172-80. doi:. https://pubmed.ncbi.nlm.nih.gov/16919859/
4. Zhao, Mengli, Chen, Jiajin, Chen, Hongyu, Zhang, Jingdong, Li, Dan. 2024. Aldo-keto reductases 7A subfamily: A mini review. In Chemico-biological interactions, 391, 110896. doi:10.1016/j.cbi.2024.110896. https://pubmed.ncbi.nlm.nih.gov/38301882/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen