C57BL/6JCya-Sec13em1flox/Cya
Common Name:
Sec13-flox
Product ID:
S-CKO-00857
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sec13-flox
Strain ID
CKOCMP-110379-Sec13-B6J-VA
Gene Name
Product ID
S-CKO-00857
Gene Alias
1110003H02Rik; Sec13l1; Sec13r
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sec13em1flox/Cya mice (Catalog S-CKO-00857) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032440
NCBI RefSeq
NM_024206
Target Region
Exon 2~3
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Sec13, as a component of the coat protein complex II (COPII), the nuclear pore complex (NPC), and the Seh1-associated (SEA)/GATOR nutrient-sensing complex, plays crucial roles in protein trafficking from the endoplasmic reticulum to the Golgi apparatus, NPC functions, and nutrient-sensing pathways [2,5]. It is also involved in processes like cellular metabolism, growth, and immune regulation [3,4]. Genetic models, such as gene-knockout models, are valuable for studying its functions.
In oligodendrocyte (OL) lineage cells, ablation of Sec13 prevented OPC differentiation, inhibited myelination and remyelination after demyelinating injury in the central nervous system (CNS), as it altered the secretome of OLs and inhibited the secretion of pleiotrophin (PTN), an autocrine factor for OL differentiation and myelin repair [1]. In zebrafish, a sec13(sq198) mutant with a carboxyl-terminus-truncated Sec13 that lost affinity to Sec31a led to ER structure disintegration in various differentiated cells, activating an unfolded protein response, cell-cycle arrest, and apoptosis, ultimately arresting the growth of developing digestive organs [6]. In mice with low-level Sec13 expression (Sec13(H/-)), specific immunological defects were observed, including low levels of MHC I and II in macrophages, low INF-γ and IL-6 in stimulated T cells, and high levels of TGF-β, indicating Sec13 as a negative modulator of TGF-β expression [4].
In conclusion, Sec13 is essential for multiple biological processes. Its role in OL differentiation and myelination, as well as in maintaining ER integrity during organogenesis and regulating immune factors, has been revealed through model-based research. The Sec13 KO/CKO mouse models contribute to understanding diseases related to demyelination, organ development, and immune-related disorders.
References:
1. Liu, Zhixiong, Yan, Minbiao, Lei, Wanying, Wang, Zhanxiang, Zhang, Liang. . Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling. In The Journal of clinical investigation, 132, . doi:10.1172/JCI155096. https://pubmed.ncbi.nlm.nih.gov/35143418/
2. Faktorová, Drahomíra, Záhonová, Kristína, Benz, Corinna, Field, Mark C, Lukeš, Julius. 2023. Functional differentiation of Sec13 paralogues in the euglenozoan protists. In Open biology, 13, 220364. doi:10.1098/rsob.220364. https://pubmed.ncbi.nlm.nih.gov/37311539/
3. Dibble, Christian C, Cantley, Lewis C. 2015. Regulation of mTORC1 by PI3K signaling. In Trends in cell biology, 25, 545-55. doi:10.1016/j.tcb.2015.06.002. https://pubmed.ncbi.nlm.nih.gov/26159692/
4. Moreira, Thais G, Zhang, Liang, Shaulov, Lihi, Faria, Ana M C, Fontoura, Beatriz M A. 2015. Sec13 Regulates Expression of Specific Immune Factors Involved in Inflammation In Vivo. In Scientific reports, 5, 17655. doi:10.1038/srep17655. https://pubmed.ncbi.nlm.nih.gov/26631972/
5. Valenstein, Max L, Rogala, Kacper B, Lalgudi, Pranav V, Quast, Jan-Philipp, Sabatini, David M. 2022. Structure of the nutrient-sensing hub GATOR2. In Nature, 607, 610-616. doi:10.1038/s41586-022-04939-z. https://pubmed.ncbi.nlm.nih.gov/35831510/
6. Niu, Xubo, Gao, Chuan, Jan Lo, Li, Hong, Wanjin, Peng, Jinrong. 2012. Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish. In Developmental biology, 367, 197-207. doi:10.1016/j.ydbio.2012.05.004. https://pubmed.ncbi.nlm.nih.gov/22609279/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen