C57BL/6JCya-Adarb1em1flox/Cya
Common Name:
Adarb1-flox
Product ID:
S-CKO-00869
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Adarb1-flox
Strain ID
CKOCMP-110532-Adarb1-B6J-VA
Gene Name
Product ID
S-CKO-00869
Gene Alias
1700057H01Rik; Adar2; D10Bwg0447e; Red1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adarb1em1flox/Cya mice (Catalog S-CKO-00869) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000098374
NCBI RefSeq
NM_001024837
Target Region
Exon 4
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Adarb1, also known as ADAR2, is an adenosine-to-inosine (A-to-I) RNA-editing enzyme. RNA editing is a crucial post-transcriptional modification process that can affect the function of RNA molecules, influencing various biological processes such as gene expression regulation [4,5,6].
In Adarb1-knockout mice, rhythms of large populations of mRNA were attenuated, indicating its profound impact on RNA rhythms. These mice also exhibited short-period rhythms in locomotor activity and gene expression, associated with abnormal accumulation of CRY2, suggesting its role in the circadian clockwork [4]. In humans, bi-allelic variants in ADARB1 are associated with microcephaly, intellectual disability, and seizures, as these variants lead to reduced editing activity on known ADAR2 substrates [5]. Downregulated ADARB1 has been found to be involved in promoting cell metastasis in lung adenocarcinoma and ovarian cancer, and is related to shorter survival times in these cancer patients. In contrast, in glioblastoma, ADARB1 is upregulated and is involved in AKT-mediated temozolomide resistance [1,2,3].
In conclusion, Adarb1 is an essential RNA-editing enzyme that plays a significant role in biological processes such as the circadian rhythm. Its dysregulation is associated with various diseases, including cancers and neurodevelopmental disorders. The study of Adarb1-knockout mouse models has provided valuable insights into its functions in these disease-related processes, helping us to better understand the underlying mechanisms and potentially develop new therapeutic strategies.
References:
1. Wang, Xiang, Xu, Zhijie, Ren, Xinxin, Gong, Zhicheng, Yan, Yuanliang. 2019. Function of low ADARB1 expression in lung adenocarcinoma. In PloS one, 14, e0222298. doi:10.1371/journal.pone.0222298. https://pubmed.ncbi.nlm.nih.gov/31491024/
2. Lu, Can, Chen, Xi, Yan, Yuanliang, Xu, Zhijie, Peng, Jinwu. 2022. Aberrant Expression of ADARB1 Facilitates Temozolomide Chemoresistance and Immune Infiltration in Glioblastoma. In Frontiers in pharmacology, 13, 768743. doi:10.3389/fphar.2022.768743. https://pubmed.ncbi.nlm.nih.gov/35177985/
3. Zhu, Wei, Xu, Zhijie, Huang, Meiyuan, Chen, Xi, Yan, Yuanliang. 2021. Downregulated ADARB1 Facilitates Cell Proliferation, Invasion and has Effect on the Immune Regulation in Ovarian Cancer. In Frontiers in bioengineering and biotechnology, 9, 792911. doi:10.3389/fbioe.2021.792911. https://pubmed.ncbi.nlm.nih.gov/35004651/
4. Terajima, Hideki, Yoshitane, Hikari, Ozaki, Haruka, Iwasaki, Wataru, Fukada, Yoshitaka. 2016. ADARB1 catalyzes circadian A-to-I editing and regulates RNA rhythm. In Nature genetics, 49, 146-151. doi:10.1038/ng.3731. https://pubmed.ncbi.nlm.nih.gov/27893733/
5. Tan, Tiong Yang, Sedmík, Jiří, Fitzgerald, Mark P, Christodoulou, John, O'Connell, Mary A. 2020. Bi-allelic ADARB1 Variants Associated with Microcephaly, Intellectual Disability, and Seizures. In American journal of human genetics, 106, 467-483. doi:10.1016/j.ajhg.2020.02.015. https://pubmed.ncbi.nlm.nih.gov/32220291/
6. Maroofian, Reza, Sedmík, Jiří, Mazaheri, Neda, O'Connell, Mary A, Houlden, Henry. 2020. Biallelic variants in ADARB1, encoding a dsRNA-specific adenosine deaminase, cause a severe developmental and epileptic encephalopathy. In Journal of medical genetics, 58, 495-504. doi:10.1136/jmedgenet-2020-107048. https://pubmed.ncbi.nlm.nih.gov/32719099/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen