C57BL/6JCya-Amhr2em1flox/Cya
Common Name:
Amhr2-flox
Product ID:
S-CKO-00870
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Amhr2-flox
Strain ID
CKOCMP-110542-Amhr2-B6J-VA
Gene Name
Product ID
S-CKO-00870
Gene Alias
C14; Misiir; Misrii; Mrii
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Amhr2em1flox/Cya mice (Catalog S-CKO-00870) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023809
NCBI RefSeq
NM_144547
Target Region
Exon 7~11
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Amhr2, the Anti-Müllerian hormone type II receptor, is a key component in the AMH signaling pathway. AMH, a member of the Transforming Growth Factor-β family, signals through Amhr2. Amhr2 is expressed in mesenchyme adjacent to the Müllerian ducts, as well as in Sertoli, Leydig, and granulosa cells. It plays a crucial role in male differentiation by mediating the regression of Müllerian ducts in mammalian fetuses. Additionally, it is involved in various reproductive processes in both males and females, such as regulating germ cell development and folliculogenesis [1,2,3].
In mammals, Amhr2 mutations lead to Persistent Müllerian Duct Syndrome (PMDS), where affected males retain Müllerian duct-derived tissues like the uterus and oviducts [1,2,5]. In Amh mutant female mice, there is accelerated ovarian primordial follicle recruitment, suggesting Amhr2's role in regulating germ cells [1]. In Japanese flounder, amhr2-deficient mutants generated using the Nuclease technology system showed male-to-female sex reversal, indicating that Amh/Amhr2 signaling causes masculinization by inhibiting estrogen synthesis during gonadal sex differentiation [4].
In conclusion, Amhr2 is essential for normal reproductive development and function. Studies using gene-knockout models in mice and other species have revealed its key role in male sexual differentiation, germ cell regulation, and sex determination in fish. Understanding Amhr2 is crucial for comprehending the mechanisms underlying reproductive disorders such as PMDS and for further research in reproductive biology [1,2,4,5].
References:
1. Mullen, Rachel D, Ontiveros, Alejandra E, Moses, Malcolm M, Behringer, Richard R. 2019. AMH and AMHR2 mutations: A spectrum of reproductive phenotypes across vertebrate species. In Developmental biology, 455, 1-9. doi:10.1016/j.ydbio.2019.07.006. https://pubmed.ncbi.nlm.nih.gov/31301298/
2. Brunello, Franco G, Rey, Rodolfo A. 2021. AMH and AMHR2 Involvement in Congenital Disorders of Sex Development. In Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation, 16, 138-146. doi:10.1159/000518273. https://pubmed.ncbi.nlm.nih.gov/34515230/
3. di Clemente, Nathalie, Racine, Chrystèle, Pierre, Alice, Taieb, Joëlle. . Anti-Müllerian Hormone in Female Reproduction. In Endocrine reviews, 42, 753-782. doi:10.1210/endrev/bnab012. https://pubmed.ncbi.nlm.nih.gov/33851994/
4. Yamaguchi, Toshiya, Kitano, Takeshi. 2023. Amh/Amhr2 Signaling Causes Masculinization by Inhibiting Estrogen Synthesis during Gonadal Sex Differentiation in Japanese Flounder (Paralichthys olivaceus). In International journal of molecular sciences, 24, . doi:10.3390/ijms24032480. https://pubmed.ncbi.nlm.nih.gov/36768803/
5. Chen, Hong, Lin, Peng, Yuan, Xin, Chen, Ruimin. 2022. Two novel AMHR2 gene variants in monozygotic twins with persistent Müllerian duct syndrome: A case report and functional study. In Molecular genetics & genomic medicine, 10, e1999. doi:10.1002/mgg3.1999. https://pubmed.ncbi.nlm.nih.gov/35655435/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen