C57BL/6NCya-Atxn3em1flox/Cya
Common Name:
Atxn3-flox
Product ID:
S-CKO-00876
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Atxn3-flox
Strain ID
CKOCMP-110616-Atxn3-B6N-VA
Gene Name
Product ID
S-CKO-00876
Gene Alias
2210008M02Rik; ATX3; MJD1; Mjd; Sca3; ataxin-3
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Atxn3em1flox/Cya mice (Catalog S-CKO-00876) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021606
NCBI RefSeq
NM_029705
Target Region
Exon 6
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
ATXN3, the product of the ATXN3 gene, is a ubiquitin hydrolase (deubiquitinase, DUB) ubiquitously expressed in various cell types, including peripheral and neuronal tissues. It is involved in multiple cellular pathways such as proteostasis, DNA repair, transcriptional regulation, and chromatin structure regulation [2,3]. Expansion of CAG trinucleotides within the ATXN3 gene leads to an expanded polyglutamine domain in the encoded protein, associated with spinocerebellar ataxia type 3 (also known as Machado-Joseph disease), the most common dominantly inherited ataxia worldwide [2,6].
Targeted deletion of ATXN3 in cancer cells largely abolished IFN-γ-and hypoxia-induced PD-L1 expression and enhanced antitumor immunity in mice, and these effects were partially reversed by PD-L1 reconstitution. Tumoral ATXN3 suppression also improved the preclinical efficacy of checkpoint blockade antitumor immunotherapy [1]. In prostate cancer, depletion of ATXN3 decreased cell proliferation, invasion, and stem-like properties, with effects rescued by YAP overexpression [4]. In contrast, in colon adenocarcinoma, CRISPR-mediated ATXN3 deletion intensified cancer growth, while ectopic expression of Galectin-9 reversed the growth of ATXN3-null colon cancer in mice [5].
In conclusion, ATXN3 is a multifunctional protein with roles in proteostasis, DNA repair, and transcriptional regulation. Its functions are context-dependent, acting as a positive regulator of PD-L1 transcription in tumor immune evasion, promoting prostate cancer progression, yet suppressing colon cancer growth. Gene knockout models in mice have been crucial in revealing these functions in cancer-related processes, enhancing our understanding of disease mechanisms and potential therapeutic targets.
References:
1. Wang, Shengnan, Iyer, Radhika, Han, Xiaohua, Sun, Zhaolin, Fang, Deyu. 2023. CRISPR screening identifies the deubiquitylase ATXN3 as a PD-L1-positive regulator for tumor immune evasion. In The Journal of clinical investigation, 133, . doi:10.1172/JCI167728. https://pubmed.ncbi.nlm.nih.gov/38038129/
2. Hernández-Carralero, Esperanza, Quinet, Grégoire, Freire, Raimundo. 2024. ATXN3: a multifunctional protein involved in the polyglutamine disease spinocerebellar ataxia type 3. In Expert reviews in molecular medicine, 26, e19. doi:10.1017/erm.2024.10. https://pubmed.ncbi.nlm.nih.gov/39320846/
3. Hernández-Carralero, Esperanza, Cabrera, Elisa, Rodríguez-Torres, Gara, Smits, Veronique A J, Freire, Raimundo. . ATXN3 controls DNA replication and transcription by regulating chromatin structure. In Nucleic acids research, 51, 5396-5413. doi:10.1093/nar/gkad212. https://pubmed.ncbi.nlm.nih.gov/36971114/
4. Wu, Longxiang, Ou, Zhenyu, Liu, Peihua, Wang, Yongjie, Tang, Jianing. 2023. ATXN3 promotes prostate cancer progression by stabilizing YAP. In Cell communication and signaling : CCS, 21, 152. doi:10.1186/s12964-023-01073-9. https://pubmed.ncbi.nlm.nih.gov/37349820/
5. Cheng, Yang, Wang, Shengnan, Gao, Qiong, Fang, Deyu. 2024. ATXN3 functions as a tumor suppressor through potentiating galectin-9-mediated apoptosis in human colon adenocarcinoma. In The Journal of biological chemistry, 300, 107415. doi:10.1016/j.jbc.2024.107415. https://pubmed.ncbi.nlm.nih.gov/38815863/
6. Paulson, Henry. . Machado-Joseph disease/spinocerebellar ataxia type 3. In Handbook of clinical neurology, 103, 437-49. doi:10.1016/B978-0-444-51892-7.00027-9. https://pubmed.ncbi.nlm.nih.gov/21827905/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen