Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6NCya-Atxn3em1flox/Cya
Common Name:
Atxn3-flox
Product ID:
S-CKO-00876
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Atxn3-flox
Strain ID
CKOCMP-110616-Atxn3-B6N-VA
Gene Name
Atxn3
Product ID
S-CKO-00876
Gene Alias
2210008M02Rik; ATX3; MJD1; Mjd; Sca3; ataxin-3
Background
C57BL/6NCya
NCBI ID
110616
Modification
Conditional knockout
Chromosome
12
Phenotype
MGI:1099442
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Atxn3em1flox/Cya mice (Catalog S-CKO-00876) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021606
NCBI RefSeq
NM_029705
Target Region
Exon 6
Size of Effective Region
~0.6 kb
Detailed Document
Click here to download >>
Overview of Gene Research
ATXN3, the product of the ATXN3 gene, is a ubiquitin hydrolase (deubiquitinase, DUB) ubiquitously expressed in various cell types, including peripheral and neuronal tissues. It is involved in multiple cellular pathways such as proteostasis, DNA repair, transcriptional regulation, and chromatin structure regulation [2,3]. Expansion of CAG trinucleotides within the ATXN3 gene leads to an expanded polyglutamine domain in the encoded protein, associated with spinocerebellar ataxia type 3 (also known as Machado-Joseph disease), the most common dominantly inherited ataxia worldwide [2,6].

Targeted deletion of ATXN3 in cancer cells largely abolished IFN-γ-and hypoxia-induced PD-L1 expression and enhanced antitumor immunity in mice, and these effects were partially reversed by PD-L1 reconstitution. Tumoral ATXN3 suppression also improved the preclinical efficacy of checkpoint blockade antitumor immunotherapy [1]. In prostate cancer, depletion of ATXN3 decreased cell proliferation, invasion, and stem-like properties, with effects rescued by YAP overexpression [4]. In contrast, in colon adenocarcinoma, CRISPR-mediated ATXN3 deletion intensified cancer growth, while ectopic expression of Galectin-9 reversed the growth of ATXN3-null colon cancer in mice [5].

In conclusion, ATXN3 is a multifunctional protein with roles in proteostasis, DNA repair, and transcriptional regulation. Its functions are context-dependent, acting as a positive regulator of PD-L1 transcription in tumor immune evasion, promoting prostate cancer progression, yet suppressing colon cancer growth. Gene knockout models in mice have been crucial in revealing these functions in cancer-related processes, enhancing our understanding of disease mechanisms and potential therapeutic targets.

References:
1. Wang, Shengnan, Iyer, Radhika, Han, Xiaohua, Sun, Zhaolin, Fang, Deyu. 2023. CRISPR screening identifies the deubiquitylase ATXN3 as a PD-L1-positive regulator for tumor immune evasion. In The Journal of clinical investigation, 133, . doi:10.1172/JCI167728. https://pubmed.ncbi.nlm.nih.gov/38038129/
2. Hernández-Carralero, Esperanza, Quinet, Grégoire, Freire, Raimundo. 2024. ATXN3: a multifunctional protein involved in the polyglutamine disease spinocerebellar ataxia type 3. In Expert reviews in molecular medicine, 26, e19. doi:10.1017/erm.2024.10. https://pubmed.ncbi.nlm.nih.gov/39320846/
3. Hernández-Carralero, Esperanza, Cabrera, Elisa, Rodríguez-Torres, Gara, Smits, Veronique A J, Freire, Raimundo. . ATXN3 controls DNA replication and transcription by regulating chromatin structure. In Nucleic acids research, 51, 5396-5413. doi:10.1093/nar/gkad212. https://pubmed.ncbi.nlm.nih.gov/36971114/
4. Wu, Longxiang, Ou, Zhenyu, Liu, Peihua, Wang, Yongjie, Tang, Jianing. 2023. ATXN3 promotes prostate cancer progression by stabilizing YAP. In Cell communication and signaling : CCS, 21, 152. doi:10.1186/s12964-023-01073-9. https://pubmed.ncbi.nlm.nih.gov/37349820/
5. Cheng, Yang, Wang, Shengnan, Gao, Qiong, Fang, Deyu. 2024. ATXN3 functions as a tumor suppressor through potentiating galectin-9-mediated apoptosis in human colon adenocarcinoma. In The Journal of biological chemistry, 300, 107415. doi:10.1016/j.jbc.2024.107415. https://pubmed.ncbi.nlm.nih.gov/38815863/
6. Paulson, Henry. . Machado-Joseph disease/spinocerebellar ataxia type 3. In Handbook of clinical neurology, 103, 437-49. doi:10.1016/B978-0-444-51892-7.00027-9. https://pubmed.ncbi.nlm.nih.gov/21827905/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest