C57BL/6JCya-Adam33em1flox/Cya
Common Name:
Adam33-flox
Product ID:
S-CKO-00885
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Adam33-flox
Strain ID
CKOCMP-110751-Adam33-B6J-VA
Gene Name
Product ID
S-CKO-00885
Gene Alias
Adaml
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adam33em1flox/Cya mice (Catalog S-CKO-00885) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000110232
NCBI RefSeq
NM_033615
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
ADAM33, also known as A Disintegrin And Metalloprotease Domain 33, is a gene with significant implications in respiratory-related biological processes. It may play a role in airway remodeling, smooth muscle cell proliferation, and angiogenesis [1,4,6]. Its restricted expression to mesenchymal cells suggests its involvement in the structural airway components of asthma [6].
Multiple studies have explored ADAM33 polymorphisms in relation to diseases. Meta-analyses have found that certain polymorphisms such as T2, Q1, F + 1, and AA genotype of T + 1 in ADAM33 are associated with asthma risk, especially in Asian populations [2,8]. ADAM33 T1, T2, V4, and Q-1 polymorphisms may also be risk factors for allergic rhinitis [3]. Additionally, the F + 1 polymorphism in ADAM33 gene is associated with an increased risk of chronic obstructive pulmonary disease (COPD) in the Asian population [7]. In terms of functional studies, silencing of ADAM33 in human aortic smooth muscle cells inhibits cell migration and regulates cytokine secretion via the PI3K/AKT/mTOR pathway, contributing to the understanding of airway vascular remodeling in asthma [5].
In conclusion, ADAM33 is a gene of great importance in respiratory diseases like asthma, allergic rhinitis, and COPD. Its polymorphisms are closely associated with disease susceptibility, and functional studies, such as gene silencing experiments, have provided insights into its role in airway-related biological processes. These findings enhance our understanding of the disease mechanisms and may potentially lead to new therapeutic strategies targeting ADAM33 in these respiratory conditions.
References:
1. Sleziak, Jakub, Gawor, Antoni, Błażejewska, Marta, Antosz, Katarzyna, Gomułka, Krzysztof. 2024. ADAM33's Role in Asthma Pathogenesis: An Overview. In International journal of molecular sciences, 25, . doi:10.3390/ijms25042318. https://pubmed.ncbi.nlm.nih.gov/38396994/
2. Li, Hui-Fang, Yan, Li-Ping, Wang, Kun, Liu, Hai-Xian, Tan, Wei. 2019. Association between ADAM33 polymorphisms and asthma risk: a systematic review and meta-analysis. In Respiratory research, 20, 38. doi:10.1186/s12931-019-1006-1. https://pubmed.ncbi.nlm.nih.gov/30791911/
3. Xu, Yu, Zhang, Ji-Xiang. 2014. ADAM33 polymorphisms and susceptibility to allergic rhinitis: a meta-analysis. In European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 272, 597-605. doi:10.1007/s00405-014-3130-3. https://pubmed.ncbi.nlm.nih.gov/24908071/
4. Tripathi, Priya, Awasthi, Shally, Gao, Peisong. 2014. ADAM metallopeptidase domain 33 (ADAM33): a promising target for asthma. In Mediators of inflammation, 2014, 572025. doi:10.1155/2014/572025. https://pubmed.ncbi.nlm.nih.gov/24817794/
5. Yan, Fang, Hu, Xin, He, Long, Hao, Yanyan, Wang, Jing. 2022. ADAM33 Silencing Inhibits Vascular Smooth Muscle Cell Migration and Regulates Cytokine Secretion in Airway Vascular Remodeling via the PI3K/AKT/mTOR Pathway. In Canadian respiratory journal, 2022, 8437348. doi:10.1155/2022/8437348. https://pubmed.ncbi.nlm.nih.gov/36091328/
6. Holgate, Stephen T, Yang, Youwen, Haitchi, Hans-Michael, Cakebread, Julie, Davies, Donna E. . The genetics of asthma: ADAM33 as an example of a susceptibility gene. In Proceedings of the American Thoracic Society, 3, 440-3. doi:. https://pubmed.ncbi.nlm.nih.gov/16799089/
7. Feng, Hong-Hong, Mao, Lu, Pan, Kai, Zhang, Ling, Rui, Dong-Sheng. 2019. Association between F+1 polymorphism in a disintegrin and metalloprotease 33 (ADAM33) gene and chronic obstructive pulmonary disease susceptibility: An evidence-based meta-analysis. In Gene, 719, 144009. doi:10.1016/j.gene.2019.144009. https://pubmed.ncbi.nlm.nih.gov/31357020/
8. Liang, Siqiao, Wei, Xuan, Gong, Chen, Chen, Zhangrong, Deng, Jingmin. 2013. A disintegrin and metalloprotease 33 (ADAM33) gene polymorphisms and the risk of asthma: a meta-analysis. In Human immunology, 74, 648-57. doi:10.1016/j.humimm.2013.01.025. https://pubmed.ncbi.nlm.nih.gov/23380143/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen