C57BL/6JCya-Adgrv1em1flox/Cya
Common Name:
Adgrv1-flox
Product ID:
S-CKO-00887
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Adgrv1-flox
Strain ID
CKOCMP-110789-Adgrv1-B6J-VA
Gene Name
Product ID
S-CKO-00887
Gene Alias
Frings; Gpr98; Mass1; Mgr1; VLGR1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adgrv1em1flox/Cya mice (Catalog S-CKO-00887) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000095585
NCBI RefSeq
NM_054053
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Adgrv1, also known as GPR98, MASS1, and VLGR1, encodes adhesion G protein-coupled receptor-V1. It is involved in synaptic function [2]. The ankle-link complex (ALC), which contains Adgrv1 along with USH2A, WHRN, and PDZD7, is crucial for hair cell development [1]. Adgrv1 may also be associated with regulating autophagy, as it interacts with key autophagosome components [3].
In a mouse model with the Adgrv1 Y6236fsX1 mutation (mimicking the human Y6244fsX1 deafness-associated mutation), the mutation disrupts the interaction between Adgrv1 and other ALC components. This leads to stereocilia disorganization and mechanoelectrical transduction (MET) deficits. Adgrv1 normally inhibits WHRN phosphorylation through regional cAMP-PKA signaling, regulating USH2A ubiquitination and stability. The mutant Adgrv1 fails to do so [1].
In conclusion, Adgrv1 plays essential roles in hair cell development through its involvement in the ALC and in regulating autophagy. The study of Adgrv1 knockout or conditional knockout mouse models, like the Adgrv1 Y6236fsX1 mutant mice, has provided insights into its role in conditions such as deafness, highlighting its significance in understanding the underlying mechanisms of related diseases [1,3].
References:
1. Guan, Ying, Du, Hai-Bo, Yang, Zhao, Sun, Jin-Peng, Xu, Zhi-Gang. 2023. Deafness-Associated ADGRV1 Mutation Impairs USH2A Stability through Improper Phosphorylation of WHRN and WDSUB1 Recruitment. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2205993. doi:10.1002/advs.202205993. https://pubmed.ncbi.nlm.nih.gov/37066759/
2. Zhou, Peng, Meng, Heng, Liang, Xiaoyu, Zhai, Qiongxiang, Yi, Yonghong. 2022. ADGRV1 Variants in Febrile Seizures/Epilepsy With Antecedent Febrile Seizures and Their Associations With Audio-Visual Abnormalities. In Frontiers in molecular neuroscience, 15, 864074. doi:10.3389/fnmol.2022.864074. https://pubmed.ncbi.nlm.nih.gov/35813073/
3. Linnert, Joshua, Güler, Baran E, Krzysko, Jacek, Wolfrum, Uwe. 2023. The adhesion G protein-coupled receptor VLGR1/ADGRV1 controls autophagy. In Basic & clinical pharmacology & toxicology, 133, 313-330. doi:10.1111/bcpt.13869. https://pubmed.ncbi.nlm.nih.gov/37002809/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen