C57BL/6JCya-Abca1em1flox/Cya
Common Name:
Abca1-flox
Product ID:
S-CKO-00931
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Abca1-flox
Strain ID
CKOCMP-11303-Abca1-B6J-VA
Gene Name
Product ID
S-CKO-00931
Gene Alias
ABC-1; Abc1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Abca1em1flox/Cya mice (Catalog S-CKO-00931) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030010
NCBI RefSeq
NM_013454
Target Region
Exon 45~46
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Abca1, also known as ATP-binding cassette transporter A1, is a key membrane transporter. It mediates the cellular efflux of phospholipids and cholesterol to lipid-poor apolipoprotein A1 (apoA1), playing a significant role in high-density lipoprotein (HDL) metabolism and reverse cholesterol transport (RCT) [3,4,5]. This process is crucial for cholesterol homeostasis in the body and is involved in various biological processes, such as preventing lipid accumulation in cells [3,6].
In a type 2 diabetic mouse model with ABCA1 deficiency in glomerular endothelial cells (DM-ABCA1-/-mice), ABCA1 deficiency exacerbated renal lipid deposition and kidney injuries, including increased creatinine levels, severe proteinuria, mesangial matrix expansion, foot-process fusion, and more pronounced renal inflammatory injury and cell death [1]. In atherosclerotic research, ABCA1 promotes efferocytosis, the clearance of apoptotic cells by phagocytes, which is important in suppressing atherosclerosis. ABCA1 promotes efferocytosis by regulating the release and expression of relevant ligands [2].
In conclusion, Abca1 is essential for cholesterol efflux and homeostasis, playing a significant role in diseases like diabetic kidney disease and atherosclerosis. Studies using gene-knockout (KO) or conditional-knockout (CKO) mouse models, such as the DM-ABCA1-/-mice, have revealed its role in specific disease-related biological processes, providing insights into potential therapeutic targets for these diseases.
References:
1. Zhang, Junlin, Wu, Yucheng, Zhang, Jie, Wang, Yiting, Liu, Fang. 2022. ABCA1 deficiency-mediated glomerular cholesterol accumulation exacerbates glomerular endothelial injury and dysfunction in diabetic kidney disease. In Metabolism: clinical and experimental, 139, 155377. doi:10.1016/j.metabol.2022.155377. https://pubmed.ncbi.nlm.nih.gov/36521550/
2. Chen, Wujun, Li, Lu, Wang, Jie, Wang, Shuai, Xing, Dongming. 2021. The ABCA1-efferocytosis axis: A new strategy to protect against atherosclerosis. In Clinica chimica acta; international journal of clinical chemistry, 518, 1-8. doi:10.1016/j.cca.2021.02.025. https://pubmed.ncbi.nlm.nih.gov/33741356/
3. Soumian, S, Albrecht, C, Davies, A H, Gibbs, R G J. . ABCA1 and atherosclerosis. In Vascular medicine (London, England), 10, 109-19. doi:. https://pubmed.ncbi.nlm.nih.gov/16013195/
4. Phillips, Michael C. 2018. Is ABCA1 a lipid transfer protein? In Journal of lipid research, 59, 749-763. doi:10.1194/jlr.R082313. https://pubmed.ncbi.nlm.nih.gov/29305383/
5. Wang, Shuhui, Smith, Jonathan D. 2014. ABCA1 and nascent HDL biogenesis. In BioFactors (Oxford, England), 40, 547-54. doi:10.1002/biof.1187. https://pubmed.ncbi.nlm.nih.gov/25359426/
6. Wang, Yang, Guo, Min, Tang, Chao-Ke. 2023. History and Development of ABCA1. In Current problems in cardiology, 49, 102036. doi:10.1016/j.cpcardiol.2023.102036. https://pubmed.ncbi.nlm.nih.gov/37595859/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen