C57BL/6JCya-Abcg1em1flox/Cya
Common Name:
Abcg1-flox
Product ID:
S-CKO-00935
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Abcg1-flox
Strain ID
CKOCMP-11307-Abcg1-B6J-VA
Gene Name
Product ID
S-CKO-00935
Gene Alias
Abc8; White
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Abcg1em1flox/Cya mice (Catalog S-CKO-00935) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024829
NCBI RefSeq
NM_009593
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Abcg1, an ATP-binding cassette half transporter, is crucial for facilitating the efflux of excess cholesterol from macrophages and other cells [2]. It is part of the reverse cholesterol transport (RCT) pathway, where it contributes to the continued cholesterol efflux to high-density lipoprotein (HDL) for its maturation, thus maintaining cellular cholesterol homeostasis [1]. This function is vital as excessive cholesterol accumulation in cells is toxic and is a key factor in diseases like atherosclerotic cardiovascular disease [1]. Genetic models, such as KO or CKO mouse models, can provide in-depth insights into its function.
In myeloid-lineage Abcg1-deficient mice, there was an ameliorated inflammatory status in adipose tissue and reduced insulin resistance. Abcg1-deficient adipose tissue macrophages (ATMs) showed a less inflammatory phenotype, modified fatty acid (FA) metabolism, and a shift in FA pools, redirecting saturated FAs from membrane phospholipids to lipid droplets [3]. These findings from the mouse model demonstrate that Abcg1 plays a role in orchestrating ATM plasticity and insulin resistance in diet-induced obesity (DIO) by rewiring saturated FA pools [3].
In conclusion, Abcg1 is essential for maintaining cellular cholesterol homeostasis through its role in cholesterol efflux. The use of Abcg1-deficient mouse models has revealed its significance in adipose tissue inflammation and insulin resistance, providing valuable insights into its function in metabolic diseases such as DIO [3].
References:
1. Yu, Xiao-Hua, Tang, Chao-Ke. . ABCA1, ABCG1, and Cholesterol Homeostasis. In Advances in experimental medicine and biology, 1377, 95-107. doi:10.1007/978-981-19-1592-5_7. https://pubmed.ncbi.nlm.nih.gov/35575923/
2. Ito, Toshimitsu. . Physiological function of ABCG1. In Drug news & perspectives, 16, 490-2. doi:. https://pubmed.ncbi.nlm.nih.gov/14668945/
3. Dahik, Veronica D, Kc, Pukar, Materne, Clément, Venteclef, Nicolas, Le Goff, Wilfried. 2024. ABCG1 orchestrates adipose tissue macrophage plasticity and insulin resistance in obesity by rewiring saturated fatty acid pools. In Science translational medicine, 16, eadi6682. doi:10.1126/scitranslmed.adi6682. https://pubmed.ncbi.nlm.nih.gov/39661702/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen