C57BL/6JCya-Chrna7em1flox/Cya
Common Name:
Chrna7-flox
Product ID:
S-CKO-00980
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Chrna7-flox
Strain ID
CKOCMP-11441-Chrna7-B6J-VA
Gene Name
Product ID
S-CKO-00980
Gene Alias
Acra7; alpha7; nAchR
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chrna7em1flox/Cya mice (Catalog S-CKO-00980) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032738
NCBI RefSeq
NM_007390
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
CHRNA7, the gene encoding the α7 neuronal nicotinic acetylcholine receptor, is ubiquitously expressed in the central nervous system and periphery. It is involved in cholinergic neurotransmission, and its function is crucial for normal cognitive processes. The regulation of CHRNA7 is complex, with a partial duplication resulting in the chimeric CHRFAM7A gene that exerts dominant-negative regulation [1].
In animal models, Chrna7 deficient mice have been studied. One study found that they did not show consistent neuropsychiatric and behavioral phenotypes related to those seen in humans, suggesting mouse behavior and EEG recordings may not be sensitive to decreased Chrna7 copy number [2]. Another study showed that Chrna7 knock-out (KO) mice display depression-like behaviors, and splenic nerve denervation can attenuate these behaviors via the spleen-gut-brain axis, indicating the splenic nerve contributes to depression-like phenotypes in these KO mice [3].
In conclusion, CHRNA7 plays a significant role in cholinergic neurotransmission and is associated with multiple disorders such as schizophrenia, bipolar disorder, and Alzheimer's disease. Mouse models, especially Chrna7 KO mice, have provided insights into the gene's role in depression-like behaviors, though their overall utility in mimicking human neuropsychiatric phenotypes remains somewhat limited.
References:
1. Sinkus, Melissa L, Graw, Sharon, Freedman, Robert, Lester, Henry A, Leonard, Sherry. 2015. The human CHRNA7 and CHRFAM7A genes: A review of the genetics, regulation, and function. In Neuropharmacology, 96, 274-88. doi:10.1016/j.neuropharm.2015.02.006. https://pubmed.ncbi.nlm.nih.gov/25701707/
2. Yin, Jiani, Chen, Wu, Yang, Hongxing, Xue, Mingshan, Schaaf, Christian P. 2017. Chrna7 deficient mice manifest no consistent neuropsychiatric and behavioral phenotypes. In Scientific reports, 7, 39941. doi:10.1038/srep39941. https://pubmed.ncbi.nlm.nih.gov/28045139/
3. Yang, Yong, Eguchi, Akifumi, Mori, Chisato, Hashimoto, Kenji. 2024. Splenic nerve denervation attenuates depression-like behaviors in Chrna7 knock-out mice via the spleen-gut-brain axis. In Journal of affective disorders, 362, 114-125. doi:10.1016/j.jad.2024.06.091. https://pubmed.ncbi.nlm.nih.gov/38944290/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen