C57BL/6JCya-Crip3em1flox/Cya
Common Name:
Crip3-flox
Product ID:
S-CKO-00990
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Crip3-flox
Strain ID
CKOCMP-114570-Crip3-B6J-VA
Gene Name
Product ID
S-CKO-00990
Gene Alias
TLP; TLP-A; TLP-B; Tlmp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Crip3em1flox/Cya mice (Catalog S-CKO-00990) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113465
NCBI RefSeq
NM_053250
Target Region
Exon 3~4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Crip3, a member of the cysteine-rich intestinal protein (CRIP) family, belongs to the Lin-1, Isl1, Mec3/double zinc finger protein family. The CRIP family plays important roles in cellular epithelial-mesenchymal transition, cell death, and tumor progression, and participates in multiple signaling pathways [1].
In terms of disease-related findings, a rare-variant association analysis revealed an association between Crip3 and age-related hearing loss, suggesting that rare-variants in Crip3 may play a role in the etiology of this condition [2]. Meta-analysis of RNA-seq data under oxidative stress conditions showed that Crip3 was downregulated, indicating a possible relationship between oxidative stress and zinc homeostasis [3]. In Xenopus laevis embryogenesis, Crip3 was expressed in the cranial ganglions and the heart, suggesting its potential role in embryonic development [4]. In prostate cancer, a methylation panel including Crip3 was significantly associated with biochemical recurrence and could prognosticate the initiation of salvage radiotherapy and/or hormone therapy after radical prostatectomy [5]. In heart failure, Crip3 was identified as a novel contributor to the pathogenesis [6]. An 8-gene HPV-related prognostic model for squamous cell carcinoma of the head and neck included Crip3 [7]. In a study on blood pressure, a genetic association was detected between pulse pressure and a locus near Crip3 [8]. For prostate cancer patients on active surveillance, a 3-marker panel consisting of miR-24, miR-30c and Crip3 methylation was significant for predicting patient reclassification [9]. In lung squamous cell carcinoma, Crip3 was among the markers specifically hypermethylated in the high-methylation subgroup, which was related to prognosis [10].
In conclusion, Crip3 is involved in various biological processes such as embryonic development, and is associated with multiple diseases including age-related hearing loss, prostate cancer, heart failure, squamous cell carcinoma of the head and neck, and lung squamous cell carcinoma. The study of Crip3 in these contexts helps to understand the molecular mechanisms underlying these diseases and may provide potential targets for diagnosis and treatment.
References：
1. Ye, Xilin, Song, Qianben, Zhang, Lumiao, Fu, Yu, Yan, Wei. 2025. Cysteine-rich intestinal protein family: structural overview, functional diversity, and roles in human disease. In Cell death discovery, 11, 114. doi:10.1038/s41420-025-02395-y. https://pubmed.ncbi.nlm.nih.gov/40118853/
2. Cornejo-Sanchez, Diana M, Li, Guangyou, Fabiha, Tabassum, DeWan, Andrew T, Leal, Suzanne M. 2023. Rare-variant association analysis reveals known and new age-related hearing loss genes. In European journal of human genetics : EJHG, 31, 638-647. doi:10.1038/s41431-023-01302-2. https://pubmed.ncbi.nlm.nih.gov/36788145/
3. Suzuki, Takayuki, Ono, Yoko, Bono, Hidemasa. 2021. Comparison of Oxidative and Hypoxic Stress Responsive Genes from Meta-Analysis of Public Transcriptomes. In Biomedicines, 9, . doi:10.3390/biomedicines9121830. https://pubmed.ncbi.nlm.nih.gov/34944646/
4. Hempel, Annemarie, Kühl, Susanne J. . Comparative expression analysis of cysteine-rich intestinal protein family members crip1, 2 and 3 during Xenopus laevis embryogenesis. In The International journal of developmental biology, 58, 841-9. doi:10.1387/ijdb.140270sk. https://pubmed.ncbi.nlm.nih.gov/26154325/
5. Savio, Andrea J, Kamdar, Shivani, Jeyapala, Renu, van der Kwast, Theodorus, Bapat, Bharati. 2019. Methylation Markers in Prostate Biopsies Are Prognosticators for Late Biochemical Recurrence and Therapy after Surgery in Prostate Cancer Patients. In The Journal of molecular diagnostics : JMD, 22, 30-39. doi:10.1016/j.jmoldx.2019.08.003. https://pubmed.ncbi.nlm.nih.gov/31605802/
6. Lee, Sang Eun, Joo, Jeong Ho, Hwang, Hee Sang, Lee, Jeong Seok, Torkamani, Ali. 2025. Spatial transcriptional landscape of human heart failure. In European heart journal, , . doi:10.1093/eurheartj/ehaf272. https://pubmed.ncbi.nlm.nih.gov/40335066/
7. Mei, Zhang, Zhengdong, Luo, Shupeng, Liu, Lei, Wang, Wang, Chuanxin. . Identification of an 8 HPV-related RNA signature as a novel prognostic biomarker for squamous cell carcinoma of the head and neck. In Medicine, 103, e36448. doi:10.1097/MD.0000000000036448. https://pubmed.ncbi.nlm.nih.gov/38335428/
8. Ganesh, Santhi K, Chasman, Daniel I, Larson, Martin G, Psaty, Bruce M, Chakravarti, Aravinda. 2014. Effects of long-term averaging of quantitative blood pressure traits on the detection of genetic associations. In American journal of human genetics, 95, 49-65. doi:10.1016/j.ajhg.2014.06.002. https://pubmed.ncbi.nlm.nih.gov/24975945/
9. Zhao, Fang, Vesprini, Danny, Liu, Richard S C, Liu, Stanley K, Bapat, Bharati. 2019. Combining urinary DNA methylation and cell-free microRNA biomarkers for improved monitoring of prostate cancer patients on active surveillance. In Urologic oncology, 37, 297.e9-297.e17. doi:10.1016/j.urolonc.2019.01.031. https://pubmed.ncbi.nlm.nih.gov/30777394/
10. Hata, Atsushi, Nakajima, Takahiro, Matsusaka, Keisuke, Yoshino, Ichiro, Kaneda, Atsushi. 2019. A low DNA methylation epigenotype in lung squamous cell carcinoma and its association with idiopathic pulmonary fibrosis and poorer prognosis. In International journal of cancer, 146, 388-399. doi:10.1002/ijc.32532. https://pubmed.ncbi.nlm.nih.gov/31241180/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen