C57BL/6JCya-Adh5em1flox/Cya
Common Name
Adh5-flox
Product ID
S-CKO-01064
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-11532-Adh5-B6J-VA
Status
When using this mouse strain in a publication, please cite “Adh5-flox Mouse (Catalog S-CKO-01064) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Adh5-flox
Strain ID
CKOCMP-11532-Adh5-B6J-VA
Gene Name
Product ID
S-CKO-01064
Gene Alias
Adh3, Adh-5, GSNOR
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000005964
NCBI RefSeq
NM_007410.3
Target Region
Exon 3~4
Size of Effective Region
~2.3 kb
Overview of Gene Research
Adh5, also known as alcohol dehydrogenase 5 (class III), chi polypeptide, is a key enzyme involved in multiple biological processes. It metabolizes S-nitrosoglutathione (GSNO) to regulate cellular S-nitrosothiol (SNO) levels, playing a crucial role in maintaining redox and nitroso-redox homeostasis. This function is associated with pathways like the stimulator of interferon genes (STING)-dependent innate immune response and the regulation of brown adipose tissue (BAT) thermogenesis [1,2].
In genetic models, Adh5 deficiency in mice leads to defective STING-dependent immune responses upon microbial challenge, facilitating viral replication, which reveals its importance in host defense against pathogens [1]. In BAT, loss of Adh5 impairs cold-induced UCP1-dependent thermogenesis and worsens obesity-associated metabolic dysfunction, indicating its role in regulating adipose thermogenesis and metabolic homeostasis [2].
In conclusion, Adh5 is essential for maintaining SNO homeostasis, which is crucial for STING-mediated innate immune responses against pathogens and for regulating adipose thermogenesis and metabolic homeostasis. Studies using Adh5 knockout mouse models have provided valuable insights into its role in these disease-relevant biological processes.
References：
1. Jia, Mutian, Chai, Li, Wang, Jie, Jia, Jihui, Zhao, Wei. 2024. S-nitrosothiol homeostasis maintained by ADH5 facilitates STING-dependent host defense against pathogens. In Nature communications, 15, 1750. doi:10.1038/s41467-024-46212-z. https://pubmed.ncbi.nlm.nih.gov/38409248/
2. Sebag, Sara C, Zhang, Zeyuan, Qian, Qingwen, Bartelt, Alexander, Yang, Ling. . ADH5-mediated NO bioactivity maintains metabolic homeostasis in brown adipose tissue. In Cell reports, 37, 110003. doi:10.1016/j.celrep.2021.110003. https://pubmed.ncbi.nlm.nih.gov/34788615/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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