C57BL/6JCya-Adnpem1flox/Cya
Common Name:
Adnp-flox
Product ID:
S-CKO-01069
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Adnp-flox
Strain ID
CKOCMP-11538-Adnp-B6J-VA
Gene Name
Product ID
S-CKO-01069
Gene Alias
mKIAA0784
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adnpem1flox/Cya mice (Catalog S-CKO-01069) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000088001
NCBI RefSeq
NM_009628
Target Region
Exon 4
Size of Effective Region
~5.3 kb
Detailed Document
Overview of Gene Research
ADNP, also known as Activity Dependent Neuroprotective Protein, is a vital gene for mammalian brain formation [5]. It plays a role in chromatin remodeling as it is associated with proteins like HP1, BRG1, and in murine stem cells, with CHD4 in a ChAHP complex [4]. Additionally, ADNP is involved in the autophagy pathway and has functions related to cytoskeletal proteins [5].
Loss-of-function experiments, such as Adnp +/- mice (a form of gene knockout), show that they suffer from learning and memory deficiencies, muscle weakness, and communication problems [2]. In zebrafish, adnp gene disruption leads to defective neurogenesis and reduced Wnt signaling [1]. Mechanistically, ADNP is required for neural induction and differentiation by enhancing Wnt signaling. It stabilizes β -Catenin through binding, preventing its degradation, and down -regulation of key neuroectoderm developmental genes [1].
In conclusion, ADNP is essential for neural development, with its functions revealed through gene knockout models in mice and zebrafish. Mutations in ADNP are linked to the Helsmoortel -Van der Aa syndrome, also known as ADNP syndrome, which is characterized by features like autism, intellectual disability, and multiple comorbidities, highlighting the importance of ADNP in understanding these neurodevelopmental disorders [1,2,3].
References:
1. Sun, Xiaoyun, Peng, Xixia, Cao, Yuqin, Zhou, Yan, Sun, Yuhua. 2020. ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling. In Nature communications, 11, 2984. doi:10.1038/s41467-020-16799-0. https://pubmed.ncbi.nlm.nih.gov/32533114/
2. Gozes, Illana. 2020. The ADNP Syndrome and CP201 (NAP) Potential and Hope. In Frontiers in neurology, 11, 608444. doi:10.3389/fneur.2020.608444. https://pubmed.ncbi.nlm.nih.gov/33329371/
3. Van Dijck, Anke, Vulto-van Silfhout, Anneke T, Cappuyns, Elisa, Vandeweyer, Geert, Kooy, R Frank. 2018. Clinical Presentation of a Complex Neurodevelopmental Disorder Caused by Mutations in ADNP. In Biological psychiatry, 85, 287-297. doi:10.1016/j.biopsych.2018.02.1173. https://pubmed.ncbi.nlm.nih.gov/29724491/
4. D'Incal, Claudio Peter, Van Rossem, Kirsten Esther, De Man, Kevin, Vanden Berghe, Wim, Kooy, R Frank. 2023. Chromatin remodeler Activity-Dependent Neuroprotective Protein (ADNP) contributes to syndromic autism. In Clinical epigenetics, 15, 45. doi:10.1186/s13148-023-01450-8. https://pubmed.ncbi.nlm.nih.gov/36945042/
5. Sragovich, Shlomo, Merenlender-Wagner, Avia, Gozes, Illana. 2017. ADNP Plays a Key Role in Autophagy: From Autism to Schizophrenia and Alzheimer's Disease. In BioEssays : news and reviews in molecular, cellular and developmental biology, 39, . doi:10.1002/bies.201700054. https://pubmed.ncbi.nlm.nih.gov/28940660/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen