C57BL/6JCya-Adss2em1flox/Cya
Common Name:
Adss2-flox
Product ID:
S-CKO-01089
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Adss2-flox
Strain ID
CKOCMP-11566-Adss2-B6J-VA
Gene Name
Product ID
S-CKO-01089
Gene Alias
AS; Adss; adSS 2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adss2em1flox/Cya mice (Catalog S-CKO-01089) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000016105
NCBI RefSeq
NM_007422
Target Region
Exon 2~4
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Adss2, adenylosuccinate synthase 2, is one of the two isozymes of adenylosuccinate synthetase which catalyzes a key step in AMP synthesis. It is widely distributed in various tissues in pigs [1]. In cardiac myocytes, electrical stimulation represses the expression of Adss2, while activating the muscle-specific Adss1 isoform [4].
In pigs, a single nucleotide polymorphism (SNP, T/C(70)) in the ninth intron of Adss2 gene was significantly associated with average daily gain trait and loin muscle area trait [1]. In bladder cancer, SNP-SNP interactions located in ST7L-Adss2 were identified, some of which were positively or negatively associated with BC risk in different ethnic groups [2]. Also, alleles near Adss2 gene showed significant parent-of-origin effects in childhood asthma, with the maternally inherited G-allele at rs3003214 potentially contributing to the maternal effect in childhood asthma [3]. In tamoxifen-resistant invasive lobular carcinoma cells, the expression of Adss2 was increased, along with other nucleic acid biosynthesis enzymes [5].
In summary, Adss2 is involved in nucleotide biosynthesis and has associations with various traits and diseases such as growth-related traits in pigs, bladder cancer, childhood asthma, and tamoxifen-resistant invasive lobular carcinoma. These findings from different genetic association studies help to understand the role of Adss2 in specific biological processes and disease conditions.
References:
1. Li, Xinyun, Zhu, Zhengmao, Mo, Delin, Zhao, Shuhong, Li, Kui. 2007. Comparative molecular characterization of ADSS1 and ADSS2 genes in pig (Sus scrofa). In Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 147, 271-7. doi:. https://pubmed.ncbi.nlm.nih.gov/17347008/
2. Yu, Evan Yi-Wen, Tang, Qiu-Yi, Chen, Ya-Ting, Zeegers, Maurice P, Wesselius, Anke. 2023. Genome-wide exploration of genetic interactions for bladder cancer risk. In International journal of cancer, 154, 81-93. doi:10.1002/ijc.34690. https://pubmed.ncbi.nlm.nih.gov/37638657/
3. Lee, Yunsung, Gjerdevik, Miriam, Jugessur, Astanand, Håberg, Siri Eldevik, Magnus, Per. . Parent-of-Origin Effects in Childhood Asthma at Seven Years of Age. In Genetic epidemiology, 49, e70007. doi:10.1002/gepi.70007. https://pubmed.ncbi.nlm.nih.gov/40133993/
4. Xia, Y, McMillin, J B, Lewis, A, Williams, R S, Kellems, R E. . Electrical stimulation of neonatal cardiac myocytes activates the NFAT3 and GATA4 pathways and up-regulates the adenylosuccinate synthetase 1 gene. In The Journal of biological chemistry, 275, 1855-63. doi:. https://pubmed.ncbi.nlm.nih.gov/10636885/
5. Gupta, Annapurna, Choueiry, Fouad, Reardon, Jesse, Ramaswamy, Bhuvaneswari, Majumder, Sarmila. 2025. Invasive lobular carcinoma integrated multi-omics analysis reveals silencing of Arginosuccinate synthase and upregulation of nucleotide biosynthesis in tamoxifen resistance. In bioRxiv : the preprint server for biology, , . doi:10.1101/2025.01.16.633236. https://pubmed.ncbi.nlm.nih.gov/39868332/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen