C57BL/6JCya-Avilem1flox/Cya
Common Name:
Avil-flox
Product ID:
S-CKO-01090
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Avil-flox
Strain ID
CKOCMP-11567-Avil-B6J-VA
Gene Name
Product ID
S-CKO-01090
Gene Alias
Advil; DOC6
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Avilem1flox/Cya mice (Catalog S-CKO-01090) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026500
NCBI RefSeq
NM_009635
Target Region
Exon 3~4
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Avil, also known as advillin, is an actin-binding protein. It plays a role in many cytoskeletal functions and is involved in critical cell infrastructure. It may function as a converging node upstream of oncogenic pathways like PAX3-FOXO1 and RAS [1,2].
In rhabdomyosarcoma (RMS), AVIL is overexpressed in many RMS cell lines, patient-derived xenograft models, and clinical samples of both alveolar (ARMS) and embryonal (ERMS) subtypes. Genetic modulation experiments show that overexpression of AVIL in mesenchymal stem cells induces neoplastic transformation both in vitro and in vivo, and reversing overexpression reverses the transformation. Silencing AVIL in RMS cells nearly eradicates them in culture and inhibits in vivo xenograft growth in mice, while gain-of-function manipulations lead to increased cell growth and migration [1,2].
In conclusion, Avil is a key actin-binding protein with important functions in cytoskeletal regulation. In the context of RMS, studies using genetic modulation (akin to loss-of-function and gain-of-function models) have revealed its critical role in tumorigenesis and maintenance, suggesting it could be a potential target for treating this pediatric soft-tissue cancer.
References:
1. Cornelison, Robert, Marrah, Laine, Fierti, Adelaide, Lynch, Sarah, Li, Hui. 2023. The Potential for Targeting AVIL and Other Actin-Binding Proteins in Rhabdomyosarcoma. In International journal of molecular sciences, 24, . doi:10.3390/ijms241814196. https://pubmed.ncbi.nlm.nih.gov/37762498/
2. Xie, Zhongqiu, Janczyk, Pawel L, Shi, Xinrui, Barr, Frederic G, Li, Hui. 2022. Rhabdomyosarcomas are oncogene addicted to the activation of AVIL. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2118048119. doi:10.1073/pnas.2118048119. https://pubmed.ncbi.nlm.nih.gov/37146302/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen