C57BL/6JCya-Glaem1flox/Cya
Common Name
Gla-flox
Product ID
S-CKO-01100
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-11605-Gla-B6J-VA
Status
When using this mouse strain in a publication, please cite “Gla-flox Mouse (Catalog S-CKO-01100) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Gla-flox
Strain ID
CKOCMP-11605-Gla-B6J-VA
Gene Name
Product ID
S-CKO-01100
Gene Alias
Ags
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr X
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000239113
NCBI RefSeq
NM_013463
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Overview of Gene Research
Gla, also known as gamma-carboxyglutamic acid, is an amino acid residue that is crucial for the function of many proteins, especially those that are vitamin K-dependent [2,3]. These Gla-containing proteins play significant roles in regulating pathological vascular calcification, with their functions strongly relying on vitamin K [2]. They are involved in various biological processes such as angiogenesis, tumorigenesis, and the body's response to inflammation [1,2,4].
In a zebrafish gla -/- mutant model of Fabry disease, proteomic analysis revealed downregulation of lysosome and mitochondrial-related proteins in renal tissues, along with disturbed energy-related pathways [5]. Abnormal mitochondrial shape, disrupted cristae morphology, and lower antioxidant activity were also observed, suggesting novel Gb3-independent pathogenic mechanisms in Fabry disease [5].
In conclusion, Gla-containing proteins have diverse functions in regulating physiological and pathological processes. The zebrafish gla -/- mutant model has been valuable in uncovering Gb3-independent mechanisms in Fabry disease, potentially leading to new therapeutic interventions for this disorder [5].
References：
1. Gheorghe, Simona Roxana, Crăciun, Alexandra Mărioara. 2016. Matrix Gla protein in tumoral pathology. In Clujul medical (1957), 89, 319-21. doi:10.15386/cjmed-579. https://pubmed.ncbi.nlm.nih.gov/27547048/
2. Galunska, Bistra, Yotov, Yoto, Nikolova, Miglena, Angelov, Atanas. 2024. Extrahepatic Vitamin K-Dependent Gla-Proteins-Potential Cardiometabolic Biomarkers. In International journal of molecular sciences, 25, . doi:10.3390/ijms25063517. https://pubmed.ncbi.nlm.nih.gov/38542487/
3. Price, P A. . Gla-containing proteins of bone. In Connective tissue research, 21, 51-7; discussion 57-60. doi:. https://pubmed.ncbi.nlm.nih.gov/2691199/
4. D'Helft, Jelle, Caccialanza, Riccardo, Derbyshire, Emma, Maes, Michael. 2022. Relevance of ω-6 GLA Added to ω-3 PUFAs Supplements for ADHD: A Narrative Review. In Nutrients, 14, . doi:10.3390/nu14163273. https://pubmed.ncbi.nlm.nih.gov/36014778/
5. Elsaid, Hassan Osman Alhassan, Rivedal, Mariell, Skandalou, Eleni, Marti, Hans-Peter, Furriol, Jessica. 2023. Proteomic analysis unveils Gb3-independent alterations and mitochondrial dysfunction in a gla-/- zebrafish model of Fabry disease. In Journal of translational medicine, 21, 591. doi:10.1186/s12967-023-04475-y. https://pubmed.ncbi.nlm.nih.gov/37670295/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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