C57BL/6JCya-Akap1em1flox/Cya
Common Name:
Akap1-flox
Product ID:
S-CKO-01120
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Akap1-flox
Strain ID
CKOCMP-11640-Akap1-B6J-VA
Gene Name
Product ID
S-CKO-01120
Gene Alias
AKAP121; AKAP84; Akap; DAKAP1; S-AKAP84
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Akap1em1flox/Cya mice (Catalog S-CKO-01120) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018572
NCBI RefSeq
NM_001042541
Target Region
Exon 2
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Akap1, also known as A-kinase anchoring protein 1, is a mitochondrial protein encoded by the Akap1 gene. It is localized in the outer mitochondrial membrane and plays a crucial role in regulating mitochondrial homeostasis. Akap1 is involved in multiple cellular processes by anchoring protein kinase A (PKA), thus regulating PKA-mediated phosphorylation and related signaling pathways [3].
In diabetic kidney disease, up-regulated Akap1 activates PKC signaling, phosphorylates Larp1, reduces TFAM expression, and impairs mtDNA replication, leading to mitochondrial dysfunction and podocyte injury. Knocking down Akap1 can rescue these impairments [1].
In diabetic cardiomyopathy, Akap1 deficiency exacerbates cardiac dysfunction, mitochondrial respiratory impairment, and cardiomyocyte apoptosis through NDUFS1-mediated mitochondrial dysfunction. Restoring Akap1 expression alleviates diabetic cardiomyopathy [2].
In diet-induced obesity, Akap1 knockout mice are resistant to obesity, as Akap1 inhibits fatty acid β-oxidation and thermogenesis in brown adipocytes [4].
In conclusion, Akap1 plays a significant role in maintaining mitochondrial function and is involved in various disease conditions such as diabetes-related kidney and heart diseases, as well as obesity. Gene knockout mouse models have been crucial in revealing its functions in these biological processes and disease mechanisms, providing potential therapeutic targets for these diseases.
References:
1. Feng, Jun, Chen, Zhaowei, Ma, Yiqiong, Liang, Wei, Ding, Guohua. 2022. AKAP1 contributes to impaired mtDNA replication and mitochondrial dysfunction in podocytes of diabetic kidney disease. In International journal of biological sciences, 18, 4026-4042. doi:10.7150/ijbs.73493. https://pubmed.ncbi.nlm.nih.gov/35844803/
2. Qi, Bingchao, He, Linjie, Zhao, Ya, Li, Yan, Ji, Lele. 2020. Akap1 deficiency exacerbates diabetic cardiomyopathy in mice by NDUFS1-mediated mitochondrial dysfunction and apoptosis. In Diabetologia, 63, 1072-1087. doi:10.1007/s00125-020-05103-w. https://pubmed.ncbi.nlm.nih.gov/32072193/
3. Marin, Wenwen. 2019. A-kinase anchoring protein 1 (AKAP1) and its role in some cardiovascular diseases. In Journal of molecular and cellular cardiology, 138, 99-109. doi:10.1016/j.yjmcc.2019.11.154. https://pubmed.ncbi.nlm.nih.gov/31783032/
4. Ji, Lele, Zhao, Ya, He, Linjie, Huang, Qichao, Xing, Jinliang. 2021. AKAP1 Deficiency Attenuates Diet-Induced Obesity and Insulin Resistance by Promoting Fatty Acid Oxidation and Thermogenesis in Brown Adipocytes. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 8, 2002794. doi:10.1002/advs.202002794. https://pubmed.ncbi.nlm.nih.gov/33747723/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen