C57BL/6JCya-Aldh2em1flox/Cya
Common Name:
Aldh2-flox
Product ID:
S-CKO-01134
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Aldh2-flox
Strain ID
CKOCMP-11669-Aldh2-B6J-VA
Gene Name
Product ID
S-CKO-01134
Gene Alias
AHD-M1; ALDH-E2; ALDHI; Ahd-5; Ahd5
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Aldh2em1flox/Cya mice (Catalog S-CKO-01134) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031411
NCBI RefSeq
NM_009656
Target Region
Exon 2~4
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Aldh2, short for aldehyde dehydrogenase 2, is a non-cytochrome P450 mitochondrial aldehyde oxidizing enzyme. Its key function is to oxidize acetaldehyde, a metabolite from alcohol drinking, into acetate. This process is crucial in alcohol metabolism and also impacts the metabolism of other endogenous and exogenous aldehydes, especially under oxidative stress [2].
In mouse models, Aldh2-deficient mice show increased lipid accumulation in the liver, which can be reversed by further Aldh2 depletion, indicating its role in alcohol-induced fatty liver regulation [1]. Aldh2-/-mice and Aldh2rs671 gene knock-in mice exhibit pulmonary and circulating NETosis in sepsis models, suggesting its importance in septic ARDS [3]. In aortic aneurysm/dissection (AAD) models, inhibition of Aldh2 retards AAD development by suppressing vascular smooth muscle cell (VSMC) phenotypical switch [4]. Aldh2-deficient mice are more susceptible to CCl4 plus alcohol-associated liver fibrosis and HCC development, as they produce harmful oxidized mitochondrial DNA-enriched extracellular vesicles that activate oncogenic pathways in HCC cells [5]. Aldh2-knockout mice are more sensitive to binge alcohol-induced gut leakiness, endotoxemia, and acute liver injury through the gut-liver axis [6]. Transplanting bone marrow from APOE-/-ALDH2-/-to APOE-/-mice increases atherosclerosis plaques due to defective macrophage efferocytosis [7].
In conclusion, Aldh2 plays essential roles in multiple biological processes related to alcohol metabolism, inflammation, and disease development. Gene knockout and knock-in mouse models have significantly contributed to understanding its functions in diseases such as alcoholic fatty liver, septic ARDS, AAD, liver cancer, alcohol-associated gut and liver injury, and atherosclerosis.
References:
1. Yao, Pengbo, Zhang, Zhenxi, Liu, Hongchao, Li, Wei, Du, Wenjing. 2023. p53 protects against alcoholic fatty liver disease via ALDH2 inhibition. In The EMBO journal, 42, e112304. doi:10.15252/embj.2022112304. https://pubmed.ncbi.nlm.nih.gov/36825429/
2. Chen, Che-Hong, Ferreira, Julio C B, Mochly-Rosen, Daria. . ALDH2 and Cardiovascular Disease. In Advances in experimental medicine and biology, 1193, 53-67. doi:10.1007/978-981-13-6260-6_3. https://pubmed.ncbi.nlm.nih.gov/31368097/
3. Xu, Changchang, Zhang, Lin, Xu, Shaoyu, Pang, Jiaojiao, Chen, Yuguo. 2024. Neutrophil ALDH2 is a new therapeutic target for the effective treatment of sepsis-induced ARDS. In Cellular & molecular immunology, 21, 510-526. doi:10.1038/s41423-024-01146-w. https://pubmed.ncbi.nlm.nih.gov/38472357/
4. Yang, Kehui, Ren, Jun, Li, Xin, Chen, Yuguo, Xu, Feng. . Prevention of aortic dissection and aneurysm via an ALDH2-mediated switch in vascular smooth muscle cell phenotype. In European heart journal, 41, 2442-2453. doi:10.1093/eurheartj/ehaa352. https://pubmed.ncbi.nlm.nih.gov/32428930/
5. Seo, Wonhyo, Gao, Yanhang, He, Yong, Niu, Junqi, Gao, Bin. 2019. ALDH2 deficiency promotes alcohol-associated liver cancer by activating oncogenic pathways via oxidized DNA-enriched extracellular vesicles. In Journal of hepatology, 71, 1000-1011. doi:10.1016/j.jhep.2019.06.018. https://pubmed.ncbi.nlm.nih.gov/31279903/
6. Rungratanawanich, Wiramon, Lin, Yuhong, Wang, Xin, Chidambaram, Saravana Babu, Song, Byoung-Joon. 2022. ALDH2 deficiency increases susceptibility to binge alcohol-induced gut leakiness, endotoxemia, and acute liver injury in mice through the gut-liver axis. In Redox biology, 59, 102577. doi:10.1016/j.redox.2022.102577. https://pubmed.ncbi.nlm.nih.gov/36528936/
7. Zhang, Jian, Zhao, Xiangkai, Guo, Yunyun, Chen, Yuguo, Xu, Feng. 2022. Macrophage ALDH2 (Aldehyde Dehydrogenase 2) Stabilizing Rac2 Is Required for Efferocytosis Internalization and Reduction of Atherosclerosis Development. In Arteriosclerosis, thrombosis, and vascular biology, 42, 700-716. doi:10.1161/ATVBAHA.121.317204. https://pubmed.ncbi.nlm.nih.gov/35354308/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen