C57BL/6JCya-Alkem1flox/Cya
Common Name:
Alk-flox
Product ID:
S-CKO-01147
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Alk-flox
Strain ID
CKOCMP-11682-Alk-B6J-VA
Gene Name
Product ID
S-CKO-01147
Gene Alias
CD246; Tcrz
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Alkem1flox/Cya mice (Catalog S-CKO-01147) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086639
NCBI RefSeq
NM_007439
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase. Its dysregulation, such as through gene rearrangements, point mutations, or deletions, has oncogenic potential across various tumour types [1]. ALK fusions, like the prevalent EML4-ALK in non-small cell lung cancer (NSCLC), lack normal spatial and temporal regulation, influencing dimerisation and substrate specificity [1].
ALK gene rearrangements are potent oncogenic drivers in malignancies like NSCLC. The discovery of ALK tyrosine kinase inhibitors (TKIs) has improved outcomes for ALK-mutated NSCLC patients, but resistance, both intrinsic and acquired, remains a challenge [2]. Different ALK TKIs are associated with distinct resistance mutation patterns. For instance, the frequency of the ALKG1202R mutation increases after treatment with second-generation agents, and secondary ALK mutations predict sensitivity to the third-generation inhibitor lorlatinib [3]. Sequential use of ALK inhibitors can also select for lorlatinib-resistant compound ALK mutations [4].
In conclusion, ALK is a key oncogenic driver in multiple cancers, especially NSCLC. Research on ALK and its inhibitors has significantly advanced treatment for ALK-positive patients. Understanding ALK's role in tumour biology through functional studies, including those on resistance mechanisms, is crucial for further optimising therapeutic strategies for ALK-positive diseases.
References:
1. Hallberg, B, Palmer, R H. . The role of the ALK receptor in cancer biology. In Annals of oncology : official journal of the European Society for Medical Oncology, 27 Suppl 3, iii4-iii15. doi:10.1093/annonc/mdw301. https://pubmed.ncbi.nlm.nih.gov/27573755/
2. Poei, Darin, Ali, Sana, Ye, Shirley, Hsu, Robert. 2024. ALK inhibitors in cancer: mechanisms of resistance and therapeutic management strategies. In Cancer drug resistance (Alhambra, Calif.), 7, 20. doi:10.20517/cdr.2024.25. https://pubmed.ncbi.nlm.nih.gov/38835344/
3. Gainor, Justin F, Dardaei, Leila, Yoda, Satoshi, Engelman, Jeffrey A, Shaw, Alice T. 2016. Molecular Mechanisms of Resistance to First- and Second-Generation ALK Inhibitors in ALK-Rearranged Lung Cancer. In Cancer discovery, 6, 1118-1133. doi:. https://pubmed.ncbi.nlm.nih.gov/27432227/
4. Yoda, Satoshi, Lin, Jessica J, Lawrence, Michael S, Hata, Aaron N, Shaw, Alice T. 2018. Sequential ALK Inhibitors Can Select for Lorlatinib-Resistant Compound ALK Mutations in ALK-Positive Lung Cancer. In Cancer discovery, 8, 714-729. doi:10.1158/2159-8290.CD-17-1256. https://pubmed.ncbi.nlm.nih.gov/29650534/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen