C57BL/6JCya-Prelpem1flox/Cya
Common Name:
Prelp-flox
Product ID:
S-CKO-01151
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Prelp-flox
Strain ID
CKOCMP-116847-Prelp-B6J-VA
Gene Name
Product ID
S-CKO-01151
Gene Alias
7330409J17Rik; SLRR2A
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prelpem1flox/Cya mice (Catalog S-CKO-01151) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000048432
NCBI RefSeq
NM_054077
Target Region
Exon 2
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
PRELP, the proline-and arginine-rich end leucine-rich repeat protein, is a small leucine-rich proteoglycan (SLRP) that binds different types of collagen [3]. It plays a role in various biological processes such as cell-cell adhesion, and is involved in regulating extracellular matrix (ECM)-related functions. It has been associated with multiple signaling pathways including the wnt/β-catenin, NF-κB, and integrin-related pathways, and is of great importance in understanding fibrosis, cancer, and neurovascular integrity [1,2,4-8]. Genetic models, especially knockout (KO) mouse models, have been crucial in studying its functions.
In fibrosis, Prelp-knockdown in cardiac and liver myofibroblasts reduced collagen expression, indicating its role in promoting collagen production in fibrotic hearts and livers [1]. In retinoblastoma, mRNA expression profiling of PRELP-/-mouse retina and PRELP-treated RB cells showed that loss of PRELP reduces cell-cell adhesion and facilitates EMT, suggesting its potential as a treatment strategy [2]. In the context of the blood-brain barrier, Prelp-/-mice presented with neuroinflammation, reduced neurovasculature integrity, and suppressed adhesion junction protein expression, highlighting PRELP's role in maintaining endothelial cell-cell integrity [4].
In conclusion, PRELP has diverse essential biological functions, including regulating collagen production in fibrosis, cell-cell adhesion in cancer, and neurovascular integrity. The use of Prelp KO mouse models has significantly contributed to understanding its role in diseases such as fibrosis, retinoblastoma, and neurovascular-related disorders, providing potential therapeutic targets for these conditions.
References:
1. Yamauchi, Yuto, Mieno, Hiroki, Suetsugu, Haruna, Watanabe, Hayato, Nakaya, Michio. 2024. Elevated PRELP expression in heart and liver fibrosis promotes collagen production. In Biochemical and biophysical research communications, 734, 150785. doi:10.1016/j.bbrc.2024.150785. https://pubmed.ncbi.nlm.nih.gov/39369540/
2. Hopkins, Jack, Asada, Ken, Leung, Alex, Sagoo, Mandeep S, Ohnuma, Shin-Ichi. 2022. PRELP Regulates Cell-Cell Adhesion and EMT and Inhibits Retinoblastoma Progression. In Cancers, 14, . doi:10.3390/cancers14194926. https://pubmed.ncbi.nlm.nih.gov/36230849/
3. Lewis, Marc. . PRELP, collagen, and a theory of Hutchinson-Gilford progeria. In Ageing research reviews, 2, 95-105. doi:. https://pubmed.ncbi.nlm.nih.gov/12437997/
4. Davaapil, Hongorzul, Hopkins, Jack, Bonnin, Nadia, Sagoo, Mandeep S, Ohnuma, Shin-Ichi. 2023. PRELP secreted from mural cells protects the function of blood brain barrier through regulation of endothelial cell-cell integrity. In Frontiers in cell and developmental biology, 11, 1147625. doi:10.3389/fcell.2023.1147625. https://pubmed.ncbi.nlm.nih.gov/37936982/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen