C57BL/6JCya-Baz2aem1flox/Cya
Common Name
Baz2a-flox
Product ID
S-CKO-01152
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-116848-Baz2a-B6J-VA
Status
When using this mouse strain in a publication, please cite “Baz2a-flox Mouse (Catalog S-CKO-01152) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Baz2a-flox
Strain ID
CKOCMP-116848-Baz2a-B6J-VA
Gene Name
Product ID
S-CKO-01152
Gene Alias
Tip5, Walp3, mKIAA0314, C030005G16Rik
Background
C57BL/6JCya
Gene Full Name
bromodomain adjacent to zinc finger domain, 2A
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 10 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000045621
NCBI Transcript ID
NM_054078
Target Region
Exon 6~8
Size of Effective Region
~2.3 kb
Overview of Gene Research
BAZ2A, also known as bromodomain adjacent to zinc finger domain protein 2A, is an epigenetic regulator. It affects the transcription of ribosomal RNA and is involved in chromatin remodeling, playing a role in maintaining genome architecture and regulating gene expression. It has been associated with various biological processes and diseases, such as cancer and tissue regeneration [2,4,5,6].
In prostate cancer, BAZ2A is required for cells with a cancer stem-like state. It binds to H3K14ac-enriched chromatin regions via its bromodomain, associates with inactive enhancers, and represses genes frequently silenced in aggressive and poorly differentiated cancer. Inhibiting the BAZ2A-BRD/H3K14ac interaction impairs prostate cancer stem cells. Also, in prostate cancer, BAZ2A represses genes through its RNA-binding TAM domain in a mechanism different from rDNA silencing, by associating with TOP2A and KDM1A [1,3].
In ground-state pluripotent stem cells, BAZ2A safeguards genome architecture by limiting the invasion of active domains into repressive compartments and regulating H3K27me3 genome occupancy in a TOP2A-dependent manner [4].
In mammalian liver regeneration, in vivo CRISPR screening identified Baz2a as a regulator, and its inhibition by specific bromodomain inhibitors led to accelerated liver healing after injuries [6].
In conclusion, BAZ2A is crucial for maintaining genome architecture, regulating gene expression, and is involved in cancer development, especially in prostate cancer, and in tissue regeneration processes like liver regeneration. The study of BAZ2A through gene knockout or other loss-of-function models has provided insights into its role in these biological processes and disease conditions, potentially offering new therapeutic strategies for related diseases.
References：
1. Peña-Hernández, Rodrigo, Aprigliano, Rossana, Carina Frommel, Sandra, Bizzarro, Juliana, Santoro, Raffaella. 2021. BAZ2A-mediated repression via H3K14ac-marked enhancers promotes prostate cancer stem cells. In EMBO reports, 22, e53014. doi:10.15252/embr.202153014. https://pubmed.ncbi.nlm.nih.gov/34403195/
2. Cazzanelli, Giulia, Vedove, Andrea Dalle, Parolin, Eleonora, Caflisch, Amedeo, Lolli, Graziano. . Reevaluation of bromodomain ligands targeting BAZ2A. In Protein science : a publication of the Protein Society, 32, e4752. doi:10.1002/pro.4752. https://pubmed.ncbi.nlm.nih.gov/37574751/
3. Roganowicz, Marcin, Bär, Dominik, Bersaglieri, Cristiana, Aprigliano, Rossana, Santoro, Raffaella. 2023. BAZ2A-RNA mediated association with TOP2A and KDM1A represses genes implicated in prostate cancer. In Life science alliance, 6, . doi:10.26508/lsa.202301950. https://pubmed.ncbi.nlm.nih.gov/37184661/
4. Dalcher, Damian, Tan, Jennifer Yihong, Bersaglieri, Cristiana, Marques, Ana Claudia, Santoro, Raffaella. 2020. BAZ2A safeguards genome architecture of ground-state pluripotent stem cells. In The EMBO journal, 39, e105606. doi:10.15252/embj.2020105606. https://pubmed.ncbi.nlm.nih.gov/33433018/
5. Dalle Vedove, Andrea, Cazzanelli, Giulia, Batiste, Laurent, Caflisch, Amedeo, Lolli, Graziano. 2022. Identification of a BAZ2A-Bromodomain Hit Compound by Fragment Growing. In ACS medicinal chemistry letters, 13, 1434-1443. doi:10.1021/acsmedchemlett.2c00173. https://pubmed.ncbi.nlm.nih.gov/36105334/
6. Jia, Yuemeng, Li, Lin, Lin, Yu-Hsuan, Ready, Joseph M, Zhu, Hao. 2022. In vivo CRISPR screening identifies BAZ2 chromatin remodelers as druggable regulators of mammalian liver regeneration. In Cell stem cell, 29, 372-385.e8. doi:10.1016/j.stem.2022.01.001. https://pubmed.ncbi.nlm.nih.gov/35090595/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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