C57BL/6NCya-Mat1aem1flox/Cya
Common Name
Mat1a-flox
Product ID
S-CKO-01196
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-11720-Mat1a-B6N-VA
When using this mouse strain in a publication, please cite “Mat1a-flox Mouse (Catalog S-CKO-01196) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Mat1a-flox
Strain ID
CKOCMP-11720-Mat1a-B6N-VA
Gene Name
Product ID
S-CKO-01196
Gene Alias
Ams, MAT, SAMS, MATA1, SAMS1, AdoMet
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000225720
NCBI RefSeq
NM_133653
Target Region
Exon 5~6
Size of Effective Region
~1.2 kb
Overview of Gene Research
Mat1a, encoding S-adenosylmethionine (SAM) synthesizing isozymes MATI/III, is mainly expressed in the normal liver. MAT1A catalyzes the conversion of methionine and ATP to SAM, the principal biological methyl donor [2,4]. This process is crucial for one-carbon metabolism and intersects with epigenetic regulation [5].
In hepatocellular carcinoma (HCC), the downregulation of MAT1A, known as the MAT1A:MAT2A switch, contributes to a decrease in SAM level [2]. MAT1A-KO mice, characterized by chronic SAM deficiency, exhibit macrovesicular steatosis, mononuclear cell infiltration in periportal areas, and HCC development [2]. Also, in HCC, the CTBP1/HDAC1/HDAC2 transcriptional complex suppresses MAT1A transcription. MAT1A overexpression increases SAM levels, promoting ferroptosis of HCC cells and enhancing CD8+ T-cell cytotoxicity [3].
In non-small cell lung cancer (NSCLC), knockdown of MAT1A impedes cell proliferation and migration while enhancing apoptosis. MAT1A promotes NSCLC progression by stabilizing CCND1 to activate glycolytic pathways [1]. Moreover, in liver cancer metastasis, loss of MAT1A increases matrix metalloproteinase-7 (MMP-7) expression, promoting metastasis [6].
In conclusion, Mat1a is essential for SAM synthesis, with its deficiency in KO mouse models linked to various liver-related pathologies such as HCC, steatosis, and inflammation. In NSCLC, it plays a role in cancer cell proliferation and glycolysis. Understanding Mat1a through these model-based studies provides insights into the mechanisms of cancer development, offering potential targets for therapeutic intervention in liver and lung cancers.
References：
1. Shen, Shengping, Liu, Ruili, Huang, Jiazheng, Luo, Qingquan, Liu, Ruijun. 2024. MAT1A activation of glycolysis to promote NSCLC progression depends on stabilizing CCND1. In Cell death & disease, 15, 768. doi:10.1038/s41419-024-07113-7. https://pubmed.ncbi.nlm.nih.gov/39438468/
2. Frau, Maddalena, Feo, Francesco, Pascale, Rosa M. 2013. Pleiotropic effects of methionine adenosyltransferases deregulation as determinants of liver cancer progression and prognosis. In Journal of hepatology, 59, 830-41. doi:10.1016/j.jhep.2013.04.031. https://pubmed.ncbi.nlm.nih.gov/23665184/
3. Li, Yaqin, Hu, Guoxin, Huang, Furong, Xu, Youhua, Tong, Guangdong. 2023. MAT1A Suppression by the CTBP1/HDAC1/HDAC2 Transcriptional Complex Induces Immune Escape and Reduces Ferroptosis in Hepatocellular Carcinoma. In Laboratory investigation; a journal of technical methods and pathology, 103, 100180. doi:10.1016/j.labinv.2023.100180. https://pubmed.ncbi.nlm.nih.gov/37230466/
4. Lu, Shelly C, Mato, José M. . S-adenosylmethionine in liver health, injury, and cancer. In Physiological reviews, 92, 1515-42. doi:10.1152/physrev.00047.2011. https://pubmed.ncbi.nlm.nih.gov/23073625/
5. Pham, Vanha N, Bruemmer, Kevin J, Toh, Joel D W, Nomura, Daniel K, Chang, Christopher J. 2023. Formaldehyde regulates S-adenosylmethionine biosynthesis and one-carbon metabolism. In Science (New York, N.Y.), 382, eabp9201. doi:10.1126/science.abp9201. https://pubmed.ncbi.nlm.nih.gov/37917677/
6. Fan, Wei, Cao, DuoYao, Yang, Bing, Yang, Heping, Lu, Shelly C. 2023. Hepatic prohibitin 1 and methionine adenosyltransferase α1 defend against primary and secondary liver cancer metastasis. In Journal of hepatology, 80, 443-453. doi:10.1016/j.jhep.2023.11.022. https://pubmed.ncbi.nlm.nih.gov/38086446/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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