C57BL/6JCya-Anp32aem1flox/Cya
Common Name:
Anp32a-flox
Product ID:
S-CKO-01204
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Anp32a-flox
Strain ID
CKOCMP-11737-Anp32a-B6J-VA
Gene Name
Product ID
S-CKO-01204
Gene Alias
Anp32; I1PP2A; LANP; PHAP1; pp32
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Anp32aem1flox/Cya mice (Catalog S-CKO-01204) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000085519
NCBI RefSeq
NM_009672
Target Region
Exon 2~4
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Anp32a, also known as acidic leucine-rich nuclear phosphoprotein-32A, is an essential host protein involved in multiple biological processes. It plays a role in histone acetylation, is a co-factor for influenza virus polymerase, and is associated with pathways like Wnt signaling. Genetic models such as gene knockout (KO) or conditional knockout (CKO) mouse models are valuable for studying its functions, which are crucial for understanding normal biological processes and disease mechanisms [4,1,2].
In Anp32a -deficient mouse models, Wnt signaling was hyper-activated in articular cartilage and the heart. Mechanistically, ANP32A inhibits target gene expression via histone acetylation masking. In the joint, this led to more severe osteoarthritis (OA), and in the heart, it potentially caused cardiac hypertrophy [2]. In zebrafish, over-expression of Anp32a mRNA promoted spinal cord regeneration and swimming capability after spinal cord injury (SCI), while its knockdown had the opposite effect, suggesting a positive role in neuronal regeneration [3].
In conclusion, Anp32a has diverse essential functions, including in histone modification, Wnt signaling regulation, and neuronal regeneration. The use of Anp32a KO mouse models has been instrumental in revealing its role in diseases such as osteoarthritis and cardiac disease, highlighting its importance in understanding these disease mechanisms.
References:
1. Carrique, Loïc, Fan, Haitian, Walker, Alexander P, Fodor, Ervin, Grimes, Jonathan M. 2020. Host ANP32A mediates the assembly of the influenza virus replicase. In Nature, 587, 638-643. doi:10.1038/s41586-020-2927-z. https://pubmed.ncbi.nlm.nih.gov/33208942/
2. Monteagudo, S, Cornelis, F M F, Wang, X, Meulenbelt, I, Lories, R J. 2022. ANP32A represses Wnt signaling across tissues thereby protecting against osteoarthritis and heart disease. In Osteoarthritis and cartilage, 30, 724-734. doi:10.1016/j.joca.2022.02.615. https://pubmed.ncbi.nlm.nih.gov/35227892/
3. Lee, Hung-Chieh, Lai, Wei-Lin, Lin, Cheng-Yung, Chou, Tze-Bin, Tsai, Huai-Jen. 2022. Anp32a Promotes Neuronal Regeneration after Spinal Cord Injury of Zebrafish Embryos. In International journal of molecular sciences, 23, . doi:10.3390/ijms232415921. https://pubmed.ncbi.nlm.nih.gov/36555564/
4. Yang, Xuejing, Lu, Bin, Sun, Xueqin, Wang, Qian-Fei, Huang, Zan. 2018. ANP32A regulates histone H3 acetylation and promotes leukemogenesis. In Leukemia, 32, 1587-1597. doi:10.1038/s41375-018-0010-7. https://pubmed.ncbi.nlm.nih.gov/29467488/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen