C57BL/6JCya-Asb10em1flox/Cya
Common Name:
Asb10-flox
Product ID:
S-CKO-01218
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Asb10-flox
Strain ID
CKOCMP-117590-Asb10-B6J-VA
Gene Name
Product ID
S-CKO-01218
Gene Alias
Asb-10
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Asb10em1flox/Cya mice (Catalog S-CKO-01218) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000048302
NCBI RefSeq
NM_080444
Target Region
Exon 3
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Asb10, or Ankyrin and SOCS Box-containing protein-10, is a member of the ASB family. It functions in ubiquitin-mediated degradation pathways. The ankyrin (ANK) repeats in Asb10 bind to proteins destined for degradation, while the suppressor of cytokine signaling (SOCS) box recruits ubiquitin ligase proteins to form a complex for ubiquitin transfer [2].
Mutations in Asb10 have been associated with glaucoma. In a Pakistani cohort, certain variants in Asb10 were found to be significantly associated with sporadic primary open-angle glaucoma (POAG), suggesting it may act as a risk factor [4]. However, in a Korean cohort, no significant association was found between Asb10 gene polymorphisms and normal-tension glaucoma (NTG) [3]. Also, in triple-negative breast cancer, Asb10, as an estrogen receptor ERα trans-activated E3 ligase, ubiquitylates tumor endothelial marker 8 (TEM8) for degradation. Its deficiency in this cancer type leads to a high homeostatic level of TEM8, which is involved in promoting stemness and vasculogenic mimicry capacity of breast cancer cells [1].
In conclusion, Asb10 is mainly involved in ubiquitin-mediated protein degradation. Studies on its gene variants in different ethnic groups have provided insights into its potential role in glaucoma. In triple-negative breast cancer, its function as an E3 ligase is crucial in regulating proteins related to cancer cell stemness and neovasculogenesis. These findings from various research models contribute to understanding disease mechanisms related to Asb10.
References:
1. Xu, Jiahui, Yang, Xiaoli, Deng, Qiaodan, Zhang, Lixing, Liu, Suling. 2021. TEM8 marks neovasculogenic tumor-initiating cells in triple-negative breast cancer. In Nature communications, 12, 4413. doi:10.1038/s41467-021-24703-7. https://pubmed.ncbi.nlm.nih.gov/34285210/
2. Keller, Kate E, Wirtz, Mary K. 2016. Working your SOCS off: The role of ASB10 and protein degradation pathways in glaucoma. In Experimental eye research, 158, 154-160. doi:10.1016/j.exer.2016.06.003. https://pubmed.ncbi.nlm.nih.gov/27296073/
3. Jung, Seung-Hyun, Lee, Young Chun, Lee, Mee Yon, Shin, Hye-Young. 2019. Lack of Correlation between ASB10 and Normal-tension Glaucoma in a Population from the Republic of Korea. In Current eye research, 45, 521-525. doi:10.1080/02713683.2019.1668949. https://pubmed.ncbi.nlm.nih.gov/31522561/
4. Micheal, Shazia, Ayub, Humaira, Islam, Farrah, Khan, Muhammad Imran, den Hollander, Anneke I. 2015. Variants in the ASB10 Gene Are Associated with Primary Open Angle Glaucoma. In PloS one, 10, e0145005. doi:10.1371/journal.pone.0145005. https://pubmed.ncbi.nlm.nih.gov/26713451/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen