C57BL/6JCya-Ap1g1em1flox/Cya
Common Name:
Ap1g1-flox
Product ID:
S-CKO-01225
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ap1g1-flox
Strain ID
CKOCMP-11765-Ap1g1-B6J-VA
Gene Name
Product ID
S-CKO-01225
Gene Alias
Adtg; D8Ertd374e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ap1g1em1flox/Cya mice (Catalog S-CKO-01225) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000093157
NCBI RefSeq
NM_009677
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Ap1g1, encoding gamma-1 subunit of adaptor-related protein complex 1 (AP1γ1), is crucial for the formation of clathrin-coated intracellular vesicles, mediating selective intracellular vesicular trafficking and polarized localization of somatodendritic proteins in neurons [1,2,3]. The AP1γ1-mediated adaptor complex functions at the trans-Golgi network and endosomes, and is ubiquitously expressed in eukaryotic cells [2,3]. Zebrafish and mouse models have been valuable in studying its functions [2,3].
In zebrafish, knocking out ap1g1 leads to severe morphological defect and lethality at the blastula stage. Heterozygous zebrafish show reduced fertility and morphological alterations in the brain, gonads and intestinal epithelium, with dysregulated cadherin-mediated cell adhesion [2]. In mice, a hypomorphic mutation of Ap1g1 causes abnormalities in sensory epithelial cells of the inner ear, pigmented epithelial cells of the retina, follicular epithelial cells of the thyroid gland, and the germinal epithelium of the testis [3]. In humans, de novo and bi-allelic variants in AP1G1 are associated with a neurodevelopmental disorder characterized by mild to severe intellectual disability, epilepsy, and developmental delay [1].
In conclusion, Ap1g1 is essential for normal development and function in various organisms. Studies using gene-knockout models in zebrafish and mice have revealed its role in processes such as neurodevelopment, fertility, and cell adhesion, and its association with human neurodevelopmental disorders. These models provide valuable insights into the functions of Ap1g1 and potential implications for understanding related diseases.
References:
1. Usmani, Muhammad A, Ahmed, Zubair M, Magini, Pamela, Riazuddin, Sheikh, Riazuddin, Saima. 2021. De novo and bi-allelic variants in AP1G1 cause neurodevelopmental disorder with developmental delay, intellectual disability, and epilepsy. In American journal of human genetics, 108, 1330-1341. doi:10.1016/j.ajhg.2021.05.007. https://pubmed.ncbi.nlm.nih.gov/34102099/
2. Mignani, Luca, Facchinello, Nicola, Varinelli, Marco, Borsani, Giuseppe, Zizioli, Daniela. 2023. Deficiency of AP1 Complex Ap1g1 in Zebrafish Model Led to Perturbation of Neurodevelopment, Female and Male Fertility; New Insight to Understand Adaptinopathies. In International journal of molecular sciences, 24, . doi:10.3390/ijms24087108. https://pubmed.ncbi.nlm.nih.gov/37108275/
3. Johnson, Kenneth R, Gagnon, Leona H, Chang, Bo. 2016. A hypomorphic mutation of the gamma-1 adaptin gene (Ap1g1) causes inner ear, retina, thyroid, and testes abnormalities in mice. In Mammalian genome : official journal of the International Mammalian Genome Society, 27, 200-12. doi:10.1007/s00335-016-9632-0. https://pubmed.ncbi.nlm.nih.gov/27090238/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen