C57BL/6JCya-Atg5em1flox/Cya
Common Name:
Atg5-flox
Product ID:
S-CKO-01247
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Atg5-flox
Strain ID
CKOCMP-11793-Atg5-B6J-VA
Gene Name
Product ID
S-CKO-01247
Gene Alias
2010107M05Rik; 3110067M24Rik; Apg5l; Atg5l; Paddy
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atg5em1flox/Cya mice (Catalog S-CKO-01247) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000039286
NCBI RefSeq
NM_053069.6
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Atg5, short for autophagy related 5, is a key component in autophagy, a conserved intracellular degradative process. It is crucial for the formation of autophagosomes, a characteristic feature of autophagy. Atg5 binds with Atg12 and Atg16L1 to form an E3 like ligase complex necessary for autophagosome expansion. Autophagy, regulated by Atg5, plays a central role in maintaining cellular nutrient and energy homeostasis [2].
In Atg5 -deficient models, significant impacts are observed. In proximal tubule-specific autophagy-deficient mice, ablation of epithelial Atg5 genes impairs autophagy, leading to enhanced NF-κB activation, immune cell infiltration, and pro-inflammatory cytokines production in obstructed kidneys [1]. In male germ cell-specific Atg5-knockout mice, testicular autophagy activity decreases, resulting in reduced fertility, abnormal sperm development, and sperm individualization issues [3]. Myeloid cell-specific Atg5 knockout mice show reduced renal fibrosis and macrophage recruitment after certain kidney injuries, as Atg5 deficiency impairs the CCL20-CCR6 axis and inhibits M2 macrophage polarization [4].
In conclusion, Atg5 is essential for autophagy and has far-reaching impacts on multiple biological processes. Studies using Atg5 knockout or conditional knockout mouse models have revealed its role in inflammation-related diseases such as kidney inflammation, and in reproductive processes like male fertility. These models have significantly contributed to understanding Atg5's function in specific disease areas and biological processes.
References:
1. Peng, Xuan, Wang, Yating, Li, Huiyan, Zhou, Yi, Mao, Haiping. 2019. ATG5-mediated autophagy suppresses NF-κB signaling to limit epithelial inflammatory response to kidney injury. In Cell death & disease, 10, 253. doi:10.1038/s41419-019-1483-7. https://pubmed.ncbi.nlm.nih.gov/30874544/
2. Changotra, Harish, Kaur, Sargeet, Yadav, Suresh Singh, Parkash, Jyoti, Duseja, Ajay. 2022. ATG5: A central autophagy regulator implicated in various human diseases. In Cell biochemistry and function, 40, 650-667. doi:10.1002/cbf.3740. https://pubmed.ncbi.nlm.nih.gov/36062813/
3. Huang, Qian, Liu, Yunhao, Zhang, Shiyang, Hess, Rex A, Zhang, Zhibing. 2020. Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice. In Autophagy, 17, 1753-1767. doi:10.1080/15548627.2020.1783822. https://pubmed.ncbi.nlm.nih.gov/32677505/
4. Zhu, Yufeng, Tan, Jiexing, Wang, Yuanzhan, Zhu, Wei, Gong, Li. 2024. Atg5 deficiency in macrophages protects against kidney fibrosis via the CCR6-CCL20 axis. In Cell communication and signaling : CCS, 22, 223. doi:10.1186/s12964-024-01600-2. https://pubmed.ncbi.nlm.nih.gov/38594728/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen