C57BL/6JCya-Apoeem1flox/Cya
Common Name
Apoe-flox
Product ID
S-CKO-01263
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-11816-Apoe-B6J-VA
When using this mouse strain in a publication, please cite “Apoe-flox Mouse (Catalog S-CKO-01263) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Apoe-flox
Strain ID
CKOCMP-11816-Apoe-B6J-VA
Gene Name
Product ID
S-CKO-01263
Gene Alias
Apo-E
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000174064
NCBI RefSeq
NM_009696
Target Region
Exon 2~4
Size of Effective Region
~3.2 kb
Overview of Gene Research
Apoe, encoding apolipoprotein E, is a major apolipoprotein involved in lipoprotein metabolism, contributing to lipid homeostasis [2]. It is a polymorphic protein with three main alleles ε4, ε3, and ε2 at a single locus, which are associated with various biological processes and diseases.
The APOE ε4 allele is the strongest genetic risk factor for sporadic Alzheimer's disease, while the ε2 allele is the strongest genetic protective factor [1]. In Alzheimer's disease, APOE pathogenesis involves amyloid-β peptide aggregation and clearance, tau neurofibrillary degeneration, microglia and astrocyte responses, and blood-brain barrier disruption [1].
In primary dyslipidemia, apoE4 is associated with increased LDL-cholesterol levels and higher cardiovascular risk, while apoE2 may be associated with a mild decrease in LDL-cholesterol levels. Some rare APOE gene variants are related to different types of dyslipidemias [2].
In glaucoma, APOE4 and APOE gene deletion (APOE-/ -) can reduce retinal ganglion cell (RGC) loss, while APOE3 and the overall presence of APOE genes (APOE + / + ) increase the risk of glaucoma RGC death [3].
In conclusion, Apoe plays a crucial role in lipid metabolism and is associated with multiple diseases such as Alzheimer's disease, primary dyslipidemia, and glaucoma. Studies on Apoe, including those using gene knockout models, help us understand its role in these disease-related biological processes, providing potential directions for disease prevention and treatment.
References：
1. Serrano-Pozo, Alberto, Das, Sudeshna, Hyman, Bradley T. . APOE and Alzheimer's disease: advances in genetics, pathophysiology, and therapeutic approaches. In The Lancet. Neurology, 20, 68-80. doi:10.1016/S1474-4422(20)30412-9. https://pubmed.ncbi.nlm.nih.gov/33340485/
2. Khalil, Yara Abou, Rabès, Jean-Pierre, Boileau, Catherine, Varret, Mathilde. 2021. APOE gene variants in primary dyslipidemia. In Atherosclerosis, 328, 11-22. doi:10.1016/j.atherosclerosis.2021.05.007. https://pubmed.ncbi.nlm.nih.gov/34058468/
3. Yi, Wenhua, Lv, De, Sun, Yue, Mu, Jingyu, Lu, Xuejing. 2023. Role of APOE in glaucoma. In Biochemical and biophysical research communications, 694, 149414. doi:10.1016/j.bbrc.2023.149414. https://pubmed.ncbi.nlm.nih.gov/38145596/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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