C57BL/6JCya-Aqp3em1flox/Cya
Common Name:
Aqp3-flox
Product ID:
S-CKO-01270
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Aqp3-flox
Strain ID
CKOCMP-11828-Aqp3-B6J-VA
Gene Name
Product ID
S-CKO-01270
Gene Alias
AQP-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Aqp3em1flox/Cya mice (Catalog S-CKO-01270) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000055327
NCBI RefSeq
NM_016689
Target Region
Exon 2~6
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Aqp3, short for aquaporin 3 (Gill blood group), is an aquaglyceroporin belonging to the AQP family. It transports water, glycerol, small solutes, and hydrogen peroxide across the plasma membrane. Aqp3 is involved in various regulatory pathways, such as the PDPK1-AKT-MTOR axis, AMPK/SIRT1, and PPAR-γ/NF-κB axes, and is crucial for numerous biological processes including cell proliferation, migration, invasion, and redox regulation [1,2,3]. Genetic models, like KO/CKO mouse models, are valuable for studying its functions.
In hepatocellular carcinoma cells, FBXW5-mediated degradation of Aqp3 suppresses autophagic cell death through the PDPK1-AKT-MTOR axis, indicating Aqp3's role in tumor cell behavior regulation [1]. In a mouse model of cholelithiasis, Aqp3 was significantly down-regulated. Overexpression of Aqp3 activated the AMPK/SIRT1 signaling pathway, reducing inflammatory injury of gallbladder mucosal epithelial cells and curbing gallstone formation [2]. In lung adenocarcinoma, Aqp3 promoted M2 macrophage polarization via the PPAR-γ/NF-κB axis, affecting tumor growth and migration. Aqp3 knockout mice models further revealed its role in mediating M2 macrophage polarization and modulating tumor glucose metabolism [3].
In conclusion, Aqp3 is essential for multiple biological functions, especially in processes related to cell behavior and redox regulation. Model-based research, particularly KO/CKO mouse models, has provided insights into its role in diseases such as hepatocellular carcinoma, cholelithiasis, and lung adenocarcinoma, offering potential therapeutic targets for these conditions.
References:
1. Liang, Yupei, Chen, Ping, Wang, Shiwen, Wei, Wenyi, Jia, Lijun. 2024. SCFFBXW5-mediated degradation of AQP3 suppresses autophagic cell death through the PDPK1-AKT-MTOR axis in hepatocellular carcinoma cells. In Autophagy, 20, 1984-1999. doi:10.1080/15548627.2024.2353497. https://pubmed.ncbi.nlm.nih.gov/38726865/
2. Wang, Ganggang, Zhang, Hao, Zhou, Zhijie, Ma, Zenghui, Wang, Xiaoliang. 2023. AQP3-mediated activation of the AMPK/SIRT1 signaling pathway curtails gallstone formation in mice by inhibiting inflammatory injury of gallbladder mucosal epithelial cells. In Molecular medicine (Cambridge, Mass.), 29, 116. doi:10.1186/s10020-023-00712-8. https://pubmed.ncbi.nlm.nih.gov/37641009/
3. Lin, Guofu, Lin, Lanlan, Chen, Xiaohui, Zeng, Yiming, Xu, Yuan. 2024. PPAR-γ/NF-kB/AQP3 axis in M2 macrophage orchestrates lung adenocarcinoma progression by upregulating IL-6. In Cell death & disease, 15, 532. doi:10.1038/s41419-024-06919-9. https://pubmed.ncbi.nlm.nih.gov/39060229/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen