C57BL/6JCya-Arf3em1flox/Cya
Common Name:
Arf3-flox
Product ID:
S-CKO-01284
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Arf3-flox
Strain ID
CKOCMP-11842-Arf3-B6J-VA
Gene Name
Product ID
S-CKO-01284
Gene Alias
5430400P17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Arf3em1flox/Cya mice (Catalog S-CKO-01284) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000053183
NCBI RefSeq
NM_007478
Target Region
Exon 3~4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Arf3, short for ADP-ribosylation factor 3, is a gene involved in diverse biological processes. It encodes a small GTPase that regulates Golgi dynamics, which is crucial for vesicle biogenesis, trafficking, and signaling via the Endoplasmic reticulum-Golgi network [2]. This process supports essential developmental processes and its disruption can lead to various disorders. Arf3 also participates in regulating key cell-signaling pathways such as AKT and ERK, which are important for cell proliferation, apoptosis, and cell-cycle regulation [1].
In cancer research, functional experiments in gastric cancer cells showed that Arf3 inhibits proliferation, induces cell-cycle arrest, and enhances apoptosis by regulating the AKT and ERK pathway [1]. In prostate cancer, ARF3 controls the modality of invasion, acting as a switch between leader cell-led chains of invasion or collective sheet movement by regulating N-cadherin levels, and its levels act as a rheostat for metastasis [4]. In osteosarcoma, circ_ARF3, a circular RNA related to Arf3, sponges miR-1299 to maintain CDK6 expression, promoting the pathogenesis of osteosarcoma [3].
In neurodevelopmental disorders, de novo missense variants in ARF3 can disrupt Golgi integrity and cause a developmental disease impairing nervous system and skeletal formation [2]. In zebrafish, disease modeling validates the dominant behavior of the mutants and their differential impact on brain and body plan formation [2]. In two unrelated children with de novo pathogenic variants in the ARF3 gene, in vitro and in vivo studies revealed that these variants impair the Golgi transport system, leading to different clinical features such as diffuse brain atrophy and cerebellar hypoplasia [5].
In conclusion, Arf3 plays a significant role in multiple biological processes and diseases. Its functions range from regulating cell proliferation, apoptosis, and cell-cycle in cancer to maintaining Golgi integrity and normal development in the nervous system and skeletal formation. Studies using cell lines, animal models like zebrafish, and patient samples have provided insights into its functions, which can potentially contribute to the understanding of disease mechanisms and the development of therapeutic strategies for related diseases such as cancer and neurodevelopmental disorders.
References:
1. Liu, Jiayun, Liu, Shenlin, Wu, Maolin, Wu, Xiaoyu, Wu, Guannan. . ARF3 inhibits proliferation and promotes apoptosis in gastric cancer by regulating AKT and ERK pathway. In Acta biochimica Polonica, 68, 223-229. doi:10.18388/abp.2020_5519. https://pubmed.ncbi.nlm.nih.gov/33847108/
2. Fasano, Giulia, Muto, Valentina, Radio, Francesca Clementina, Lauri, Antonella, Tartaglia, Marco. 2022. Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish. In Nature communications, 13, 6841. doi:10.1038/s41467-022-34354-x. https://pubmed.ncbi.nlm.nih.gov/36369169/
3. Gao, Ai-Mei, Yuan, Chunyan, Hu, Ai-Xin, Liu, Xiang-Sheng. 2020. circ_ARF3 regulates the pathogenesis of osteosarcoma by sponging miR-1299 to maintain CDK6 expression. In Cellular signalling, 72, 109622. doi:10.1016/j.cellsig.2020.109622. https://pubmed.ncbi.nlm.nih.gov/32240746/
4. Sandilands, Emma, Freckmann, Eva C, Cumming, Erin M, Blyth, Karen, Bryant, David M. 2023. The small GTPase ARF3 controls invasion modality and metastasis by regulating N-cadherin levels. In The Journal of cell biology, 222, . doi:10.1083/jcb.202206115. https://pubmed.ncbi.nlm.nih.gov/36880595/
5. Sakamoto, Masamune, Sasaki, Kazunori, Sugie, Atsushi, Miyake, Noriko, Matsumoto, Naomichi. . De novo ARF3 variants cause neurodevelopmental disorder with brain abnormality. In Human molecular genetics, 31, 69-81. doi:10.1093/hmg/ddab224. https://pubmed.ncbi.nlm.nih.gov/34346499/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen