C57BL/6NCya-Rhoaem1flox/Cya
Common Name:
Rhoa-flox
Product ID:
S-CKO-01297
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rhoa-flox
Strain ID
CKOCMP-11848-Rhoa-B6N-VA
Gene Name
Product ID
S-CKO-01297
Gene Alias
Arha; Arha1; Arha2
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Rhoaem1flox/Cya mice (Catalog S-CKO-01297) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000007959
NCBI RefSeq
NM_016802
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
RhoA, short for Ras homolog gene family member A, is a small GTPase of the Rho family. It is a key regulator of cytoskeletal dynamics and is involved in multiple signal transduction pathways, influencing various cellular functions such as cell survival, migration, adhesion, and proliferation [1,2]. RhoA and its downstream effector proteins are highly expressed in the nervous system, suggesting a significant role in neurons and glial cells [1]. The RhoA/ROCK signaling pathway is associated with many neuronal functions and central nervous system diseases [3,4].
In neurodegenerative diseases like Parkinson's, Alzheimer's, Huntington's, and amyotrophic lateral sclerosis, aberrant RhoA signaling has been implicated [1]. In Alzheimer's disease, the RhoA/ROCK signaling pathway promotes the disease occurrence by increasing β -secretase activity, promoting amyloid-beta production, and forming neurofibrillary tangles [4]. In ischemic stroke, the RhoA/ROCK signaling pathway and astrocytes play roles in neurological function and tissue repair after brain injury [3]. In hematological cancers, deregulated RHOA activity is linked to cancer growth, progression, and metastasis, and RHOA may have both tumor-promoting and tumor-suppressive functions depending on the context [2]. In capillary malformation-arteriovenous malformation syndrome, loss of RASA1 may lead to constitutive activation of RhoA signaling, increasing vascular permeability [5]. Also, RhoA is crucial for myoblast fusion during muscle regeneration and hypertrophy, and is involved in DNA damage response, mediating cell cycle arrest [6,7].
In conclusion, RhoA is a vital regulator of numerous cellular functions and is involved in various biological processes and disease conditions. Studies on RhoA, especially those using gene knockout or conditional knockout mouse models (although not explicitly detailed in the provided abstracts but inferred from the research context), have enhanced our understanding of its functions in neurodegenerative diseases, cancers, and other disorders, providing potential therapeutic targets for these diseases.
References:
1. Schmidt, Sissel Ida, Blaabjerg, Morten, Freude, Kristine, Meyer, Morten. 2022. RhoA Signaling in Neurodegenerative Diseases. In Cells, 11, . doi:10.3390/cells11091520. https://pubmed.ncbi.nlm.nih.gov/35563826/
2. Santos, Juliana Carvalho, Profitós-Pelejà, Núria, Sánchez-Vinces, Salvador, Roué, Gaël. 2023. RHOA Therapeutic Targeting in Hematological Cancers. In Cells, 12, . doi:10.3390/cells12030433. https://pubmed.ncbi.nlm.nih.gov/36766776/
3. Lu, Weizhuo, Chen, Zhiwu, Wen, Jiyue. 2021. RhoA/ROCK signaling pathway and astrocytes in ischemic stroke. In Metabolic brain disease, 36, 1101-1108. doi:10.1007/s11011-021-00709-4. https://pubmed.ncbi.nlm.nih.gov/33745103/
4. Cai, RuoLan, Wang, YangYang, Huang, ZhenTing, Yu, Changyin, Cai, Zhiyou. 2021. Role of RhoA/ROCK signaling in Alzheimer's disease. In Behavioural brain research, 414, 113481. doi:10.1016/j.bbr.2021.113481. https://pubmed.ncbi.nlm.nih.gov/34302876/
5. Eisa-Beygi, Shahram, Vo, Nghia Jack, Link, Brian A. 2020. RhoA activation-mediated vascular permeability in capillary malformation-arteriovenous malformation syndrome: a hypothesis. In Drug discovery today, 26, 1790-1793. doi:10.1016/j.drudis.2020.12.012. https://pubmed.ncbi.nlm.nih.gov/33358701/
6. Noviello, Chiara, Kobon, Kassandra, Randrianarison-Huetz, Voahangy, Falcone, Sestina, Sotiropoulos, Athanassia. 2023. RhoA Is a Crucial Regulator of Myoblast Fusion. In Cells, 12, . doi:10.3390/cells12232673. https://pubmed.ncbi.nlm.nih.gov/38067102/
7. Cheng, Chibin, Seen, Daniel, Zheng, Chunwen, Zeng, Ruijie, Li, Enmin. 2021. Role of Small GTPase RhoA in DNA Damage Response. In Biomolecules, 11, . doi:10.3390/biom11020212. https://pubmed.ncbi.nlm.nih.gov/33546351/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen