C57BL/6JCya-Rhoaem1flox/Cya
Common Name:
Rhoa-flox
Product ID:
S-CKO-01298
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rhoa-flox
Strain ID
CKOCMP-11848-Rhoa-B6J-VA
Gene Name
Product ID
S-CKO-01298
Gene Alias
Arha; Arha1; Arha2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rhoaem1flox/Cya mice (Catalog S-CKO-01298) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000007959
NCBI RefSeq
NM_016802
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
RhoA, short for Ras homolog gene family member A, is a small GTPase of the Rho family. It is crucial in regulating multiple signal transduction pathways, influencing diverse cellular functions such as cell survival, migration, adhesion, proliferation, and cytoskeletal dynamics [1,2]. The RhoA/ROCK signaling pathway, with Rho-associated coiled-coil kinase (ROCK) being a downstream serine/threonine kinase, is involved in processes like cell migration, proliferation, and survival [3].
RhoA has been implicated in numerous diseases. In neurodegenerative diseases such as Parkinson's, Alzheimer's, Huntington's, and amyotrophic lateral sclerosis, aberrant RhoA signaling may play a role [1]. In Alzheimer's disease, the RhoA/ROCK signaling pathway can promote its occurrence, as ROCK activation increases β-secretase activity, promotes amyloid-beta (Aβ) production, and Aβ further activates ROCK [3]. In hematological cancers, deregulated RHOA activity is linked to growth, progression, and metastasis, and recent sequencing studies have found both gain and loss-of-function mutations in primary leukemia/lymphoma [2]. In capillary malformation-arteriovenous malformation (CM-AVM) syndrome, loss of RASA1 may lead to constitutive activation of RhoA signaling in endothelial cells, increasing vascular permeability [4]. In myoblast fusion, RhoA in satellite cells is indispensable for correct muscle regeneration and hypertrophy, as its absence prevents proper cell fusion [5]. Also, in DNA damage response (DDR), RhoA has a role in mediating cell cycle arrest, with components like ATM, FEN1, ROS, and Rho-specific guanine exchange factors (GEFs) regulating its activation [6].
In conclusion, RhoA is a key regulator in multiple biological processes and is involved in various disease conditions. The study of RhoA through model-based research, such as KO/CKO mouse models (not specifically mentioned in the references but generally relevant in functional gene studies), helps in understanding its role in normal and disease-related biological functions, providing potential therapeutic targets for neurodegenerative diseases, cancers, and other disorders.
References:
1. Schmidt, Sissel Ida, Blaabjerg, Morten, Freude, Kristine, Meyer, Morten. 2022. RhoA Signaling in Neurodegenerative Diseases. In Cells, 11, . doi:10.3390/cells11091520. https://pubmed.ncbi.nlm.nih.gov/35563826/
2. Santos, Juliana Carvalho, Profitós-Pelejà, Núria, Sánchez-Vinces, Salvador, Roué, Gaël. 2023. RHOA Therapeutic Targeting in Hematological Cancers. In Cells, 12, . doi:10.3390/cells12030433. https://pubmed.ncbi.nlm.nih.gov/36766776/
3. Cai, RuoLan, Wang, YangYang, Huang, ZhenTing, Yu, Changyin, Cai, Zhiyou. 2021. Role of RhoA/ROCK signaling in Alzheimer's disease. In Behavioural brain research, 414, 113481. doi:10.1016/j.bbr.2021.113481. https://pubmed.ncbi.nlm.nih.gov/34302876/
4. Eisa-Beygi, Shahram, Vo, Nghia Jack, Link, Brian A. 2020. RhoA activation-mediated vascular permeability in capillary malformation-arteriovenous malformation syndrome: a hypothesis. In Drug discovery today, 26, 1790-1793. doi:10.1016/j.drudis.2020.12.012. https://pubmed.ncbi.nlm.nih.gov/33358701/
5. Noviello, Chiara, Kobon, Kassandra, Randrianarison-Huetz, Voahangy, Falcone, Sestina, Sotiropoulos, Athanassia. 2023. RhoA Is a Crucial Regulator of Myoblast Fusion. In Cells, 12, . doi:10.3390/cells12232673. https://pubmed.ncbi.nlm.nih.gov/38067102/
6. Cheng, Chibin, Seen, Daniel, Zheng, Chunwen, Zeng, Ruijie, Li, Enmin. 2021. Role of Small GTPase RhoA in DNA Damage Response. In Biomolecules, 11, . doi:10.3390/biom11020212. https://pubmed.ncbi.nlm.nih.gov/33546351/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen