C57BL/6JCya-Bmal1em1flox/Cya
Common Name:
Bmal1-flox
Product ID:
S-CKO-01309
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Bmal1-flox
Strain ID
CKOCMP-11865-Bmal1-B6J-VA
Gene Name
Product ID
S-CKO-01309
Gene Alias
Arnt3; Arntl; BMAL1b; MOP3; bHLHe5; bmal1b'
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bmal1em1flox/Cya mice (Catalog S-CKO-01309) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000047321
NCBI RefSeq
NM_007489
Target Region
Exon 6~8
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Bmal1, also known as Brain and muscle arnt-like protein 1, is a crucial transcription factor and a core component of the circadian oscillation. It forms a heterodimer with Clock, binding to the E-box element in the promoters of genes like Per and Cry, regulating the rhythmic expression of circadian clock-controlled genes. Bmal1 is involved in maintaining immune homeostasis, and is associated with pathways related to metabolism, immunity, and reproduction, playing an overall vital role in various biological processes [2].
In disease-related studies, Bmal1 down-regulation in cardiomyocyte-specific knockout (CKB) and type 2 diabetes (T2D) mice hearts accelerates cardiac hypertrophy and diastolic dysfunction, while overexpression alleviates these pathological changes in diabetic cardiomyopathy (DCM), suggesting its role in DCM development through Bcl2/IP3R-mediated mitochondrial Ca2+ overload [1]. In Bmal1 -/- mutant mice (a model with non-functional circadian clock), colitis is more severe, with constant disease activity, impaired recovery, and loss of daily rhythms in inflammation and epithelial proliferation, indicating Bmal1's importance in regulating the daily timing of colitis [3]. In AML, knocking down BMAL1 inhibits cell growth by blocking the cell cycle and promotes ferroptosis, enhancing the efficacy of certain cancer therapeutic drugs, as BMAL1 up-regulation is observed in AML patients with poor prognosis [4].
In conclusion, Bmal1 is essential for maintaining normal physiological functions related to circadian rhythms, immune homeostasis, and metabolism. The use of gene knockout (KO) and conditional knockout (CKO) mouse models has revealed its significant roles in diseases such as DCM, colitis, and AML, providing potential therapeutic targets for these diseases.
References:
1. Zhang, Nannan, Yu, Hao, Liu, Tianzi, Hou, Xiaofeng, Zou, Jiangang. 2023. Bmal1 downregulation leads to diabetic cardiomyopathy by promoting Bcl2/IP3R-mediated mitochondrial Ca2+ overload. In Redox biology, 64, 102788. doi:10.1016/j.redox.2023.102788. https://pubmed.ncbi.nlm.nih.gov/37356134/
2. Fan, Xu-Li, Song, Ying, Qin, Dong-Xu, Lin, Pei-Yao. 2021. Regulatory Effects of Clock and Bmal1 on Circadian Rhythmic TLR Expression. In International reviews of immunology, 42, 101-112. doi:10.1080/08830185.2021.1931170. https://pubmed.ncbi.nlm.nih.gov/34544330/
3. Taleb, Zainab, Carmona-Alcocer, Vania, Stokes, Kyle, Khan, Waliul I, Karpowicz, Phillip. 2022. BMAL1 Regulates the Daily Timing of Colitis. In Frontiers in cellular and infection microbiology, 12, 773413. doi:10.3389/fcimb.2022.773413. https://pubmed.ncbi.nlm.nih.gov/35223537/
4. Zheng, Hong, Wu, Ting, Lin, Zhi, Li, Jia-Da, Yang, Minghua. 2024. Targeting BMAL1 reverses drug resistance of acute myeloid leukemia cells and promotes ferroptosis through HMGB1-GPX4 signaling pathway. In Journal of cancer research and clinical oncology, 150, 231. doi:10.1007/s00432-024-05753-y. https://pubmed.ncbi.nlm.nih.gov/38703241/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen