C57BL/6JCya-Atoh1em1flox/Cya
Common Name:
Atoh1-flox
Product ID:
S-CKO-01332
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Atoh1-flox
Strain ID
CKOCMP-11921-Atoh1-B6J-VA
Gene Name
Product ID
S-CKO-01332
Gene Alias
Hath1; MATH-1; Math1; bHLHa14
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atoh1em1flox/Cya mice (Catalog S-CKO-01332) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000101351
NCBI RefSeq
NM_007500
Target Region
Exon 1
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Atoh1, also known as Math1, Hath1, and Cath1 in mouse, human, and chicken respectively, is a proneural basic helix-loop-helix (bHLH) transcription factor. It is crucial for the development and regeneration of cochlear hair cells, differentiation of neurons, secretory cells in the gut, and mechanoreceptors [1,3,4,5,7]. Atoh1 is transcriptionally regulated by signaling pathways like Notch and Wnt, and post-translationally by the ubiquitin-proteasome pathway [1].
In Lkb1Lgr5-KO mice, where LKB1 was deleted from intestinal stem cells (ISCs), most crypts had functional ISCs with increased Atoh1 mRNA levels, leading to more cells in the secretory lineage, and this did not involve Notch or Wnt signaling [2]. In Tff1-CreERT2; Rosa26Tdtomato and Tff1-CreERT2; Apcfl/fl ; p53fl/fl (TcPP) mouse models, Atoh1 deletion in pit cells of gastric adenocarcinoma conferred stemness, accelerated cancer stemness and chemoresistance, and downregulated growth arrest-specific protein 1 (GAS1) [6].
In conclusion, Atoh1 is essential for the development and differentiation of multiple cell types including cochlear hair cells, neurons, and intestinal secretory cells. Gene knockout models in mice have revealed its role in promoting stemness and disease progression in gastric adenocarcinoma, and its regulation in intestinal stem cell fate. Understanding Atoh1's functions can potentially lead to new therapeutic strategies for diseases like deafness and gastric cancer.
References：
1. Cheng, Yen-Fu. 2017. Atoh1 regulation in the cochlea: more than just transcription. In Journal of Zhejiang University. Science. B, 20, 146-155. doi:10.1631/jzus.B1600438. https://pubmed.ncbi.nlm.nih.gov/29770645/
2. Gao, Yajing, Yan, Yan, Tripathi, Sushil, Katajisto, Pekka, Mäkelä, Tomi P. 2020. LKB1 Represses ATOH1 via PDK4 and Energy Metabolism and Regulates Intestinal Stem Cell Fate. In Gastroenterology, 158, 1389-1401.e10. doi:10.1053/j.gastro.2019.12.033. https://pubmed.ncbi.nlm.nih.gov/31930988/
3. Costa, Aida, Powell, Lynn M, Lowell, Sally, Jarman, Andrew P. 2016. Atoh1 in sensory hair cell development: constraints and cofactors. In Seminars in cell & developmental biology, 65, 60-68. doi:10.1016/j.semcdb.2016.10.003. https://pubmed.ncbi.nlm.nih.gov/27751776/
4. Hongmiao, Ren, Wei, Liu, Bing, Hu, Xiong, Ding Da, Jihao, Ren. . Atoh1: landscape for inner ear cell regeneration. In Current gene therapy, 14, 101-11. doi:. https://pubmed.ncbi.nlm.nih.gov/24611726/
5. Zhong, Chao, Fu, Yong, Pan, Wen, Yu, Jun, Wang, Jinfu. 2018. Atoh1 and other related key regulators in the development of auditory sensory epithelium in the mammalian inner ear: function and interplay. In Developmental biology, 446, 133-141. doi:10.1016/j.ydbio.2018.12.025. https://pubmed.ncbi.nlm.nih.gov/30605626/
6. Zhong, Qing, Wang, Hua-Gen, Yang, Ji-Hong, Chen, Qi-Yue, Huang, Chang-Ming. 2023. Loss of ATOH1 in Pit Cell Drives Stemness and Progression of Gastric Adenocarcinoma by Activating AKT/mTOR Signaling through GAS1. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2301977. doi:10.1002/advs.202301977. https://pubmed.ncbi.nlm.nih.gov/37824217/
7. Mulvaney, Joanna, Dabdoub, Alain. 2012. Atoh1, an essential transcription factor in neurogenesis and intestinal and inner ear development: function, regulation, and context dependency. In Journal of the Association for Research in Otolaryngology : JARO, 13, 281-93. doi:10.1007/s10162-012-0317-4. https://pubmed.ncbi.nlm.nih.gov/22370966/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen