C57BL/6JCya-Neurog3em1flox/Cya
Common Name:
Neurog3-flox
Product ID:
S-CKO-01336
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Neurog3-flox
Strain ID
CKOCMP-11925-Neurog3-B6J-VA
Gene Name
Product ID
S-CKO-01336
Gene Alias
Atoh5; Math4B; bHLHa7; ngn3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Neurog3em1flox/Cya mice (Catalog S-CKO-01336) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000050103
NCBI RefSeq
NM_009719
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Neurog3, a basic helix-loop-helix (bHLH) transcription factor, is of great significance in the development of pancreatic islet cells and enteroendocrine cells. In the pancreas, it is crucial for the generation of four major islet cell types (α, β, δ, and γ), and in the intestine, it drives the differentiation of enteroendocrine cells. Its activity is involved in cell fate determination pathways during organogenesis [1,2,3,6].
In mouse models, loss of Neurog3 led to depletion of intestinal enteroendocrine cells and an increase in goblet cells. Reduced Neurog3 gene dosage shifted enteroendocrine progenitors towards the goblet cell lineage, indicating that Neurog3 gene dosage controls the fate choice of enteroendocrine progenitors [2]. In humans, homozygous mutations of NEUROG3 manifest with neonatal or childhood diabetes. Mice lacking Neurog3 do not form pancreatic islet cells, including insulin-secreting beta cells, highlighting its essential role in pancreatic endocrine cell development [3,4,5].
In conclusion, Neurog3 is a master regulator in the development of pancreatic and intestinal endocrine cells. Studies using gene knockout (KO) mouse models have revealed its critical functions in cell fate determination and differentiation. Understanding the role of Neurog3 provides insights into the pathogenesis of diabetes and other related endocrine disorders, offering potential targets for therapeutic interventions in these disease areas [2,3,4,5].
References:
1. Liu, Jing, Banerjee, Amrita, Herring, Charles A, Lau, Ken S, Gu, Guoqiang. . Neurog3-Independent Methylation Is the Earliest Detectable Mark Distinguishing Pancreatic Progenitor Identity. In Developmental cell, 48, 49-63.e7. doi:10.1016/j.devcel.2018.11.048. https://pubmed.ncbi.nlm.nih.gov/30620902/
2. Li, Hui Joyce, Ray, Subir K, Kucukural, Alper, Gradwohl, Gerard, Leiter, Andrew B. 2020. Reduced Neurog3 Gene Dosage Shifts Enteroendocrine Progenitor Towards Goblet Cell Lineage in the Mouse Intestine. In Cellular and molecular gastroenterology and hepatology, 11, 433-448. doi:10.1016/j.jcmgh.2020.08.006. https://pubmed.ncbi.nlm.nih.gov/32822913/
3. Schreiber, Valérie, Mercier, Reuben, Jiménez, Sara, Serup, Palle, Gradwohl, Gérard. 2021. Extensive NEUROG3 occupancy in the human pancreatic endocrine gene regulatory network. In Molecular metabolism, 53, 101313. doi:10.1016/j.molmet.2021.101313. https://pubmed.ncbi.nlm.nih.gov/34352411/
4. Wejaphikul, Karn, Srilanchakon, Khomsak, Kamolvisit, Wuttichart, Porntaveetus, Thantrira, Shotelersuk, Vorasuk. . Novel Variants and Phenotypes in NEUROG3-Associated Syndrome. In The Journal of clinical endocrinology and metabolism, 108, 52-58. doi:10.1210/clinem/dgac554. https://pubmed.ncbi.nlm.nih.gov/36149814/
5. Solorzano-Vargas, R Sergio, Bjerknes, Matthew, Wang, Jiafang, Georgia, Senta, Martín, Martín G. 2020. Null mutations of NEUROG3 are associated with delayed-onset diabetes mellitus. In JCI insight, 5, . doi:10.1172/jci.insight.127657. https://pubmed.ncbi.nlm.nih.gov/31805014/
6. Spence, Jason R, Mayhew, Christopher N, Rankin, Scott A, Shroyer, Noah F, Wells, James M. 2010. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. In Nature, 470, 105-9. doi:10.1038/nature09691. https://pubmed.ncbi.nlm.nih.gov/21151107/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen