C57BL/6JCya-Atox1em1flox/Cya
Common Name:
Atox1-flox
Product ID:
S-CKO-01337
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Atox1-flox
Strain ID
CKOCMP-11927-Atox1-B6J-VA
Gene Name
Product ID
S-CKO-01337
Gene Alias
Atx1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atox1em1flox/Cya mice (Catalog S-CKO-01337) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108857
NCBI RefSeq
NM_009720
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Atox1, also known as Antioxidant 1 or antioxidant protein 1, is a copper chaperone that plays a crucial role in regulating intracellular copper ion balance [1,2,3,4,5,6,7,8,9]. It transfers copper to the copper export P-type ATPases ATP7A and ATP7B for copper excretion, and is involved in various pathways related to neurotransmitter biosynthesis, iron efflux, neovascularization, wound healing, and blood pressure regulation [7]. It also has antioxidant properties and may act as a transcription factor regulating Ccnd1 [4]. Genetic models, such as gene knockout (KO) or conditional knockout (CKO) mouse models, are valuable for studying Atox1's functions.
In a mouse model of TNBS-induced colitis, Atox1 knockout preempted the up-regulation of inflammatory cytokines, M1 polarization markers, and p47phox expression induced by TNBS, indicating Atox1 plays a pro-inflammatory role in intestinal inflammation via the ROS-NLRP3 inflammasome pathway [3]. In a traumatic brain injury (TBI) mouse model, Atox1 expression decreased after TBI. Overexpression of Atox1 in mice preserved the hippocampal structure, reduced oxidative stress, and ameliorated learning and memory impairments. In HT-22 cells with stretch injury, Atox1 overexpression mitigated oxidative stress. Knockdown of DJ-1 in HT-22 cells impaired Atox1's antioxidant capacity, suggesting DJ-1 mediates Atox1's ability to withstand oxidative stress [1].
In conclusion, Atox1 is essential for copper homeostasis and has antioxidant functions. KO/CKO mouse models have revealed its roles in intestinal inflammation and TBI. Understanding Atox1's functions through these models provides insights into potential therapeutic approaches for post-traumatic neurological dysfunction and inflammatory bowel disease [1,3].
References:
1. Zhao, Pengzhan, Shi, Wenqian, Ye, Yangfan, Wang, Xinyue, Ji, Jing. 2024. Atox1 protects hippocampal neurons after traumatic brain injury via DJ-1 mediated anti-oxidative stress and mitophagy. In Redox biology, 72, 103156. doi:10.1016/j.redox.2024.103156. https://pubmed.ncbi.nlm.nih.gov/38640584/
2. Chen, Lin, Li, Na, Zhang, Meiqi, Shan, Changliang, Wang, Jing. 2021. APEX2-based Proximity Labeling of Atox1 Identifies CRIP2 as a Nuclear Copper-binding Protein that Regulates Autophagy Activation. In Angewandte Chemie (International ed. in English), 60, 25346-25355. doi:10.1002/anie.202108961. https://pubmed.ncbi.nlm.nih.gov/34550632/
3. Chen, MingXian, Chen, Yu, Fu, Rui, Li, HaiXia, Shen, TangBiao. 2024. Atox1 regulates macrophage polarization in intestinal inflammation via ROS-NLRP3 inflammasome pathway. In Journal of translational medicine, 22, 497. doi:10.1186/s12967-024-05314-4. https://pubmed.ncbi.nlm.nih.gov/38796413/
4. Muller, Patricia A J, Klomp, Leo W J. 2008. ATOX1: a novel copper-responsive transcription factor in mammals? In The international journal of biochemistry & cell biology, 41, 1233-6. doi:10.1016/j.biocel.2008.08.001. https://pubmed.ncbi.nlm.nih.gov/18761103/
5. Xue, Qian, Kang, Rui, Klionsky, Daniel J, Liu, Jinbao, Chen, Xin. 2023. Copper metabolism in cell death and autophagy. In Autophagy, 19, 2175-2195. doi:10.1080/15548627.2023.2200554. https://pubmed.ncbi.nlm.nih.gov/37055935/
6. Hatori, Yuta, Inouye, Sachiye, Akagi, Reiko. 2017. Thiol-based copper handling by the copper chaperone Atox1. In IUBMB life, 69, 246-254. doi:10.1002/iub.1620. https://pubmed.ncbi.nlm.nih.gov/28294521/
7. Hatori, Yuta, Lutsenko, Svetlana. 2016. The Role of Copper Chaperone Atox1 in Coupling Redox Homeostasis to Intracellular Copper Distribution. In Antioxidants (Basel, Switzerland), 5, . doi:10.3390/antiox5030025. https://pubmed.ncbi.nlm.nih.gov/27472369/
8. Hatori, Yuta, Lutsenko, Svetlana. 2013. An expanding range of functions for the copper chaperone/antioxidant protein Atox1. In Antioxidants & redox signaling, 19, 945-57. doi:10.1089/ars.2012.5086. https://pubmed.ncbi.nlm.nih.gov/23249252/
9. Chen, Ji, Jiang, Yunhui, Shi, Hua, Fan, Xueying, Li, Chenghua. 2020. The molecular mechanisms of copper metabolism and its roles in human diseases. In Pflugers Archiv : European journal of physiology, 472, 1415-1429. doi:10.1007/s00424-020-02412-2. https://pubmed.ncbi.nlm.nih.gov/32506322/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen