C57BL/6JCya-Fxyd2em1flox/Cya
Common Name:
Fxyd2-flox
Product ID:
S-CKO-01342
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Fxyd2-flox
Strain ID
CKOCMP-11936-Fxyd2-B6J-VA
Gene Name
Product ID
S-CKO-01342
Gene Alias
Atp1g1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fxyd2em1flox/Cya mice (Catalog S-CKO-01342) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041005
NCBI RefSeq
NM_052823
Target Region
Exon 2~3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Fxyd2, a regulatory subunit of Na,K-ATPase, also known as the γ subunit, is crucial for adapting the functional properties of Na,K-ATPase to cellular requirements. It is involved in multiple biological processes, such as renal Mg handling, pain regulation, and cell growth, and is associated with various diseases including hypomagnesemia, chronic pain, and glioma [1,2,3]. The study of Fxyd2 through genetic models like KO mouse models can provide valuable insights into its functions.
In KO mouse models, adult Fxyd2 (-∕-) mice exhibited beneficial phenotypes. In the kidney, there was elevated activity of Na,K-ATPase and apical sodium transporters, yet the mice maintained sodium balance and normotensive state. They also showed enhanced glucose tolerance, increased circulating insulin, and no insulin resistance, along with increased beta cell proliferation and mass related to PI3K-Akt pathway activation [5]. In addition, loss of Fxyd2 function, either constitutively in Fxyd2-/- mice or acutely in neuropathic rats, alleviated mechanical hypersensitivity induced by peripheral nerve lesions, indicating its role in maintaining neuropathic pain [4].
In conclusion, Fxyd2 is essential for regulating Na,K-ATPase activity and has diverse functions in different biological processes. The study of Fxyd2 KO mouse models has contributed to understanding its role in diseases such as neuropathic pain and has revealed beneficial metabolic adaptations related to sodium balance and glucose control, providing potential therapeutic strategies.
References:
1. Mayan, Haim, Farfel, Zvi, Karlish, Steven J D. . Renal Mg handling, FXYD2 and the central role of the Na,K-ATPase. In Physiological reports, 6, e13843. doi:10.14814/phy2.13843. https://pubmed.ncbi.nlm.nih.gov/30175537/
2. Derre, Alexandre, Soler, Noelian, Billoux, Valentine, Pattyn, Alexandre, Venteo, Stephanie. 2023. FXYD2 antisense oligonucleotide provides an efficient approach for long-lasting relief of chronic peripheral pain. In JCI insight, 8, . doi:10.1172/jci.insight.161246. https://pubmed.ncbi.nlm.nih.gov/37154155/
3. Zhou, Kaijia, Jiang, Tao, Liu, Yanwei, Huang, Lijie, Li, Guanzhang. 2021. FXYD2 mRNA expression represents a new independent factor that affects survival of glioma patients and predicts chemosensitivity of patients to temozolomide. In BMC neurology, 21, 438. doi:10.1186/s12883-021-02476-2. https://pubmed.ncbi.nlm.nih.gov/34753441/
4. Ventéo, Stéphanie, Laffray, Sophie, Wetzel, Christiane, Carroll, Patrick, Pattyn, Alexandre. 2016. Fxyd2 regulates Aδ- and C-fiber mechanosensitivity and is required for the maintenance of neuropathic pain. In Scientific reports, 6, 36407. doi:10.1038/srep36407. https://pubmed.ncbi.nlm.nih.gov/27805035/
5. Arystarkhova, Elena. 2016. Beneficial Renal and Pancreatic Phenotypes in a Mouse Deficient in FXYD2 Regulatory Subunit of Na,K-ATPase. In Frontiers in physiology, 7, 88. doi:10.3389/fphys.2016.00088. https://pubmed.ncbi.nlm.nih.gov/27014088/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen