C57BL/6JCya-Bcat1em1flox/Cya
Common Name:
Bcat1-flox
Product ID:
S-CKO-01392
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Bcat1-flox
Strain ID
CKOCMP-12035-Bcat1-B6J-VA
Gene Name
Product ID
S-CKO-01392
Gene Alias
BCATc; Eca39
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bcat1em1flox/Cya mice (Catalog S-CKO-01392) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032402
NCBI RefSeq
NM_001024468
Target Region
Exon 6
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Bcat1, short for branched-chain amino acid transaminase 1, is an enzyme that initiates the catabolism of branched-chain amino acids (BCAAs) like leucine. It plays a crucial role in BCAA metabolism, which is associated with various biological processes such as providing macromolecule precursors, and is linked to important metabolic and signaling pathways [4].
In cancer, increased Bcat1 expression has been associated with aggressive phenotypes. For example, in gastric cancer, a gain-of-function mutation (Bcat1E61A) with around 2.8% enrichment in clinical samples boosts BCAA catabolism, accelerates cell growth and motility, and promotes tumor development by directly interacting with RhoC [1]. In acute myeloid leukaemia (AML), Bcat1 restricts α-ketoglutarate (αKG) levels in AML stem cells, leading to IDHmut-like DNA hypermethylation. Knockdown of Bcat1 in leukaemia cells causes αKG accumulation, resulting in growth and survival defects and abrogating leukaemia-initiating potential [2]. In glioblastoma, Bcat1 is upregulated selectively in IDH wildtype tumors. Targeting Bcat1 combined with α-ketoglutarate triggers metabolic synthetic lethality in IDHWT glioblastoma cells, as loss of Bcat1 leads to metabolic alterations that are augmented by α-ketoglutarate treatment [3].
In conclusion, Bcat1 is essential in BCAA metabolism, and its dysregulation is implicated in multiple disease conditions, especially cancers. Gene knockout and related functional studies in mouse models have been instrumental in revealing its role in promoting cell growth, motility, and tumor development in cancer, and in regulating stem-cell-related functions in AML. These findings provide potential therapeutic targets for treating such diseases.
References:
1. Qian, Lin, Li, Na, Lu, Xiao-Chen, Yin, Miao, Lei, Qun-Ying. 2023. Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression. In Nature metabolism, 5, 1159-1173. doi:10.1038/s42255-023-00818-7. https://pubmed.ncbi.nlm.nih.gov/37337119/
2. Raffel, Simon, Falcone, Mattia, Kneisel, Niclas, Radlwimmer, Bernhard, Trumpp, Andreas. 2017. BCAT1 restricts αKG levels in AML stem cells leading to IDHmut-like DNA hypermethylation. In Nature, 551, 384-388. doi:10.1038/nature24294. https://pubmed.ncbi.nlm.nih.gov/29144447/
3. Zhang, Bo, Peng, Hui, Zhou, Mi, Wang, Yingfei, Luo, Weibo. . Targeting BCAT1 Combined with α-Ketoglutarate Triggers Metabolic Synthetic Lethality in Glioblastoma. In Cancer research, 82, 2388-2402. doi:10.1158/0008-5472.CAN-21-3868. https://pubmed.ncbi.nlm.nih.gov/35499760/
4. Papathanassiu, Adonia E, Ko, Jeong-Hun, Imprialou, Martha, Mauro, Claudio, Behmoaras, Jacques. 2017. BCAT1 controls metabolic reprogramming in activated human macrophages and is associated with inflammatory diseases. In Nature communications, 8, 16040. doi:10.1038/ncomms16040. https://pubmed.ncbi.nlm.nih.gov/28699638/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen