C57BL/6JCya-Bcheem1flox/Cya
Common Name:
Bche-flox
Product ID:
S-CKO-01395
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Bche-flox
Strain ID
CKOCMP-12038-Bche-B6J-VA
Gene Name
Product ID
S-CKO-01395
Gene Alias
C730038G20Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bcheem1flox/Cya mice (Catalog S-CKO-01395) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029367
NCBI RefSeq
NM_009738
Target Region
Exon 2
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Bche, also known as butyrylcholinesterase, is an enzyme typically associated with hydrolyzing the neurotransmitter acetylcholine. It has been long considered an "orphan enzyme" with no clear physiological role, other than metabolizing exogenous bioactive esters in diet or medicines [1]. However, recent studies suggest it is involved in multiple biological processes, such as lipid metabolism, weight gain, fat metabolism, and emotional behaviors [1,2].
In gene knockout mouse models, BChE-knockout mice showed faster weight gain on high-fat diets [1]. Viral gene transfer studies in mice revealed that BChE hydrolyzes ghrelin, the "hunger hormone", affecting its circulating levels, which in turn impacts weight gain, fat metabolism, and emotional behaviors like aggression [1]. Overexpression of BChE led to low ghrelin levels, reducing aggression and social stress in mice, and in some cases, increasing lifespan in group-housed animals [1].
In conclusion, Bche plays important roles in lipid metabolism, weight and fat regulation, and emotional behaviors as revealed through mouse model-based research. Its function in hydrolyzing ghrelin has implications for understanding weight-related and emotional-behavioral disorders. Moreover, Bche has been associated with diseases such as endometrial cancer, Alzheimer's disease, and is related to the prolonged effect of muscle relaxants, indicating its significance in multiple disease areas [1,3,4,5].
References:
1. Brimijoin, Stephen, Chen, Vicky Ping, Pang, Yuan-Ping, Geng, Liyi, Gao, Yang. 2016. Physiological roles for butyrylcholinesterase: A BChE-ghrelin axis. In Chemico-biological interactions, 259, 271-275. doi:10.1016/j.cbi.2016.02.013. https://pubmed.ncbi.nlm.nih.gov/26915976/
2. Gok, Muslum, Cicek, Cigdem, Sari, Suat, Bodur, Ebru. 2022. Novel activity of human BChE: Lipid hydrolysis. In Biochimie, 204, 127-135. doi:10.1016/j.biochi.2022.09.008. https://pubmed.ncbi.nlm.nih.gov/36126749/
3. Liu, Junxiu, Tian, Tian, Liu, Xiangyu, Cui, Zhumei. 2022. BCHE as a Prognostic Biomarker in Endometrial Cancer and Its Correlation with Immunity. In Journal of immunology research, 2022, 6051092. doi:10.1155/2022/6051092. https://pubmed.ncbi.nlm.nih.gov/35915658/
4. Jasiecki, Jacek, Wasąg, Bartosz. 2019. Butyrylcholinesterase Protein Ends in the Pathogenesis of Alzheimer's Disease-Could BCHE Genotyping Be Helpful in Alzheimer's Therapy? In Biomolecules, 9, . doi:10.3390/biom9100592. https://pubmed.ncbi.nlm.nih.gov/31601022/
5. Brazzolotto, Xavier, Courcelle, Sébastien, Sauvanet, Christophe, Ceppa, Franck, Delacour, Hervé. 2020. Characterization of four BCHE mutations associated with prolonged effect of suxamethonium. In The pharmacogenomics journal, 21, 165-173. doi:10.1038/s41397-020-00192-7. https://pubmed.ncbi.nlm.nih.gov/33024248/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen