C57BL/6JCya-Bdnfem1flox/Cya
Common Name:
Bdnf-flox
Product ID:
S-CKO-01409
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Bdnf-flox
Strain ID
CKOCMP-12064-Bdnf-B6J-VA
Gene Name
Product ID
S-CKO-01409
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bdnfem1flox/Cya mice (Catalog S-CKO-01409) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000111051
NCBI RefSeq
NM_001048139
Target Region
Exon 2
Size of Effective Region
~4.6 kb
Detailed Document
Overview of Gene Research
Bdnf, short for Brain-derived neurotrophic factor, is one of the most widely distributed and studied neurotrophins in the mammalian brain. It signals through the tropomycin receptor kinase B (TrkB) and the low-affinity p75 neurotrophin receptor (p75NTR). Bdnf plays a crucial role in the growth, development, and plasticity of glutamatergic and GABAergic synapses, and influences serotonergic and dopaminergic neurotransmission via modulation of neuronal differentiation. It is also important for the transformation of synaptic activity into long-term memories and for adult neurogenesis in the hippocampus [1].
In antidepressant research, genetic manipulations disrupting BDNF signaling in mice attenuated the antidepressant-like response to conventional antidepressants. BDNF and TrkB conditional knockout mice showed abolished antidepressant-like response to ketamine, highlighting BDNF as an essential determinant of antidepressant efficacy [2]. In Parkinson's disease, although direct delivery of exogenous BDNF or gene therapy to enhance its expression did not relieve symptoms, physical training-induced BDNF increase was neuroprotective in animal models, suggesting a potential adjunctive therapy role [3].
In conclusion, Bdnf is vital for normal neuronal development, synaptic plasticity, and neurotransmission. Gene knockout and conditional knockout mouse models have revealed its significance in diseases like depression and Parkinson's disease. These models help us understand the role of Bdnf in specific biological processes and offer potential therapeutic directions for related diseases.
References:
1. Colucci-D'Amato, Luca, Speranza, Luisa, Volpicelli, Floriana. 2020. Neurotrophic Factor BDNF, Physiological Functions and Therapeutic Potential in Depression, Neurodegeneration and Brain Cancer. In International journal of molecular sciences, 21, . doi:10.3390/ijms21207777. https://pubmed.ncbi.nlm.nih.gov/33096634/
2. Björkholm, Carl, Monteggia, Lisa M. 2015. BDNF - a key transducer of antidepressant effects. In Neuropharmacology, 102, 72-9. doi:10.1016/j.neuropharm.2015.10.034. https://pubmed.ncbi.nlm.nih.gov/26519901/
3. Palasz, Ewelina, Wysocka, Adrianna, Gasiorowska, Anna, Niewiadomski, Wiktor, Niewiadomska, Grazyna. 2020. BDNF as a Promising Therapeutic Agent in Parkinson's Disease. In International journal of molecular sciences, 21, . doi:10.3390/ijms21031170. https://pubmed.ncbi.nlm.nih.gov/32050617/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen