C57BL/6JCya-Bex2em1flox/Cya
Common Name:
Bex2-flox
Product ID:
S-CKO-01411
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Bex2-flox
Strain ID
CKOCMP-12069-Bex2-B6J-VA
Gene Name
Product ID
S-CKO-01411
Gene Alias
Bex1; REX-3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bex2em1flox/Cya mice (Catalog S-CKO-01411) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000049130
NCBI RefSeq
NM_009749
Target Region
Exon 3
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Bex2, or Brain Expressed X-linked gene 2, was originally identified for promoting transcription by interacting with transcription factors in DNA-binding complexes. It is involved in multiple cellular processes and associated with several signaling pathways such as NF-κB, c-Jun/JNK, and ErbB2 pathways [2]. Bex2 has been shown to play a significant role in carcinogenesis, making it biologically important in understanding cancer development [2]. Genetic models like gene knockout (KO) could potentially be valuable in further elucidating its functions.
Knockdown of Bex2 in various cancer cell lines has revealed its role in cancer-related processes. In lung cancer cells, crotonylated Bex2 enhances mitophagy by facilitating the interaction between NDP52 and LC3B, and its overexpression inhibits chemotherapy-induced apoptosis [1]. In breast, colorectal, hepatocellular cancers, and brain tumors, Bex2 promotes cell growth, survival, migration, and invasion, and regulates apoptosis and the cell cycle through cross-talk with relevant signaling pathways [2]. In gastric and hepatocellular carcinomas, Bex2 is associated with cancer stemness and poor prognosis, and its knockdown decreases stem-like properties and increases drug sensitivity [3,4]. In colorectal cancer, Bex2 promotes cell proliferation via the JNK/c-Jun pathway [5]. In intrahepatic cholangiocarcinoma, Bex2 is essential for maintaining dormant cancer stem cells by suppressing mitochondrial activity [6]. In glioma cells, Bex2 promotes migration and invasion, likely mediated by N-cadherin and MMP-2 [7].
In conclusion, Bex2 is a gene with diverse functions in cancer biology, mainly promoting cancer-related processes such as cell growth, survival, migration, and invasion, and is associated with cancer stemness and poor prognosis in multiple cancer types. The use of knockdown and overexpression models in various cancer cell lines has significantly contributed to understanding Bex2's role in these disease conditions, providing potential therapeutic targets for cancer treatment.
References：
1. Mu, Ning, Wang, Yu, Li, Xiaopeng, Su, Ling, Liu, Xiangguo. 2023. Crotonylated BEX2 interacts with NDP52 and enhances mitophagy to modulate chemotherapeutic agent-induced apoptosis in non-small-cell lung cancer cells. In Cell death & disease, 14, 645. doi:10.1038/s41419-023-06164-6. https://pubmed.ncbi.nlm.nih.gov/37777549/
2. Naderi, Ali. 2019. Molecular functions of brain expressed X-linked 2 (BEX2) in malignancies. In Experimental cell research, 376, 221-226. doi:10.1016/j.yexcr.2019.02.014. https://pubmed.ncbi.nlm.nih.gov/30779920/
3. Yasumoto, Akihiro, Fujimori, Haruna, Mochizuki, Mai, Shibata, Chikashi, Tamai, Keiichi. 2023. BEX2 is poor prognostic factor and required for cancer stemness in gastric cancer. In Biochemical and biophysical research communications, 655, 59-67. doi:10.1016/j.bbrc.2023.03.025. https://pubmed.ncbi.nlm.nih.gov/36933308/
4. Fukushi, Daisuke, Shibuya-Takahashi, Rie, Mochizuki, Mai, Satoh, Kennichi, Tamai, Keiichi. 2021. BEX2 is required for maintaining dormant cancer stem cell in hepatocellular carcinoma. In Cancer science, 112, 4580-4592. doi:10.1111/cas.15115. https://pubmed.ncbi.nlm.nih.gov/34424582/
5. Hu, Yeting, Xiao, Qian, Chen, Haiyan, Yuan, Ying, Ding, Kefeng. 2017. BEX2 promotes tumor proliferation in colorectal cancer. In International journal of biological sciences, 13, 286-294. doi:10.7150/ijbs.15171. https://pubmed.ncbi.nlm.nih.gov/28367093/
6. Tamai, Keiichi, Nakamura-Shima, Mao, Shibuya-Takahashi, Rie, Sugamura, Kazuo, Satoh, Kennichi. 2020. BEX2 suppresses mitochondrial activity and is required for dormant cancer stem cell maintenance in intrahepatic cholangiocarcinoma. In Scientific reports, 10, 21592. doi:10.1038/s41598-020-78539-0. https://pubmed.ncbi.nlm.nih.gov/33299012/
7. Zhou, Xiuping, Xu, Xuebin, Meng, Qingming, Shi, Qiong, Yu, Rutong. 2012. Bex2 is critical for migration and invasion in malignant glioma cells. In Journal of molecular neuroscience : MN, 50, 78-87. doi:10.1007/s12031-012-9864-8. https://pubmed.ncbi.nlm.nih.gov/22907646/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen