C57BL/6JCya-Bmi1em1flox/Cya
Common Name:
Bmi1-flox
Product ID:
S-CKO-01429
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Bmi1-flox
Strain ID
CKOCMP-12151-Bmi1-B6J-VA
Gene Name
Product ID
S-CKO-01429
Gene Alias
Bmi-1; Pcgf4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bmi1em1flox/Cya mice (Catalog S-CKO-01429) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028071
NCBI RefSeq
NM_007552
Target Region
Exon 2~10
Size of Effective Region
~5.7 kb
Detailed Document
Overview of Gene Research
Bmi1, also known as B-cell-specific Moloney murine leukemia virus integration site-1, is a member of the PRC1 family and a well-recognized transcriptional suppressor. It has the ability to maintain the self-renewal and proliferation of tissue-specific stem cells. Bmi1 is involved in various cellular processes such as cell cycle progression, senescence, apoptosis, and angiogenesis. It functions through pathways like the INK4a/ARF locus, NF-κB signaling pathway, and PTEN/PI3K/AKT signaling pathway [1,2,3,5].
In the heart, emerging data show that Bmi1 is expressed in heart tissue and impacts various cardiac pathological conditions. In multiple myeloma, BMI1-KO MM-Φs from BM cells of BMI1-KO mice exhibited reduced proliferation and suppressed expression of angiogenic factors, losing their pro-myeloma effects, indicating that BMI1 mediates the pro-myeloma functions of MM-MΦs [1,4]. In spermatogonial stem cells (SSCs), BMI1-deficient SSCs showed transcriptional activation of Wnt10b and nuclear translocation of β-catenin, and suppressing Wnt/β-catenin signaling restored SSC maintenance, revealing a mechanism for BMI1 in SSC maintenance [6].
In conclusion, Bmi1 is essential for maintaining the self-renewal and proliferation of tissue-specific stem cells. Studies using KO mouse models have revealed its roles in cardiac pathological conditions, multiple myeloma, and SSC maintenance. These findings provide insights into its functions in different biological processes and potential therapeutic implications for related diseases.
References:
1. Yang, Dan, Liu, Han-Qing, Yang, Zheng, Fan, Di, Tang, Qi-Zhu. 2021. BMI1 in the heart: Novel functions beyond tumorigenesis. In EBioMedicine, 63, 103193. doi:10.1016/j.ebiom.2020.103193. https://pubmed.ncbi.nlm.nih.gov/33421944/
2. Wang, Ru, Fan, Hengwei, Sun, Ming, Lv, Zhongwei, Yi, Wanwan. . Roles of BMI1 in the Initiation, Progression, and Treatment of Hepatocellular Carcinoma. In Technology in cancer research & treatment, 21, 15330338211070689. doi:10.1177/15330338211070689. https://pubmed.ncbi.nlm.nih.gov/35072573/
3. Sahasrabuddhe, Anagh A. 2016. BMI1: A Biomarker of Hematologic Malignancies. In Biomarkers in cancer, 8, 65-75. doi:10.4137/BIC.S33376. https://pubmed.ncbi.nlm.nih.gov/27168727/
4. Zhang, Danfeng, Huang, Jingcao, Wang, Fangfang, Niu, Ting, Zheng, Yuhuan. 2021. BMI1 regulates multiple myeloma-associated macrophage's pro-myeloma functions. In Cell death & disease, 12, 495. doi:10.1038/s41419-021-03748-y. https://pubmed.ncbi.nlm.nih.gov/33993198/
5. M, Janaki Ramaiah, S, Vaishnave. 2018. BMI1 and PTEN are key determinants of breast cancer therapy: A plausible therapeutic target in breast cancer. In Gene, 678, 302-311. doi:10.1016/j.gene.2018.08.022. https://pubmed.ncbi.nlm.nih.gov/30096458/
6. Yu, Jun, Shen, Cong, Lin, Meng, Zheng, Bo, Sun, Fei. 2022. BMI1 promotes spermatogonial stem cell maintenance by epigenetically repressing Wnt10b/β-catenin signaling. In International journal of biological sciences, 18, 2807-2820. doi:10.7150/ijbs.70441. https://pubmed.ncbi.nlm.nih.gov/35541907/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen