C57BL/6JCya-C1qaem1flox/Cya
Common Name:
C1qa-flox
Product ID:
S-CKO-01472
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
C1qa-flox
Strain ID
CKOCMP-12259-C1qa-B6J-VA
Gene Name
Product ID
S-CKO-01472
Gene Alias
Adic; C1q
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-C1qaem1flox/Cya mice (Catalog S-CKO-01472) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046285
NCBI RefSeq
NM_007572
Target Region
Exon 2~3
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
C1qa, a component of the complement system, plays a pivotal role in immune responses, especially in the anti-microbial pathway within tissues [1]. It has been investigated as an invariant biomarker for tissue macrophages [1]. C1qa is also involved in gene-gene interactions and may be associated with the age-at-onset in familial amyloid polyneuropathy patients [3]. Additionally, it is related to pathways like HALLMARK_COMPLEMENT, which is correlated and activated with C1qa, and is considered a stimulated risk gene for atherosclerosis [4].
In a C1qa KO mouse model, C1qa deficiency enhanced susceptibility to mouse hepatitis virus A59 systemic infection. C1qa KO mice showed severe histopathological changes such as hepatocellular necrosis and interstitial pneumonia, along with higher viral copy numbers in organs compared to wild-type mice. This enhanced susceptibility was also associated with dramatic elevations in spleen IFN-γ, MIP-1α, and MCP-1 in C1qa KO mice, suggesting that activation of the classical complement pathway may be important for protecting the host against MHV A59 infection [2].
In conclusion, C1qa is crucial in immune-related functions, especially in anti-microbial responses and as a potential biomarker. The C1qa KO mouse model has been instrumental in revealing its role in viral infection susceptibility, providing insights into the importance of the classical complement pathway in protecting against certain viral infections like MHV A59.
References:
1. Horowitz, Amir, Yu, Haocheng, Pandey, Sonalisa, Mishra, Bud, Sahoo, Debashis. 2024. C1QA is an invariant biomarker for tissue macrophages. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.01.26.577475. https://pubmed.ncbi.nlm.nih.gov/38328228/
2. Kim, Han Woong, Seo, Sun Min, Kim, Jun Young, Lee, Han Woong, Choi, Yang Kyu. . C1qa deficiency in mice increases susceptibility to mouse hepatitis virus A59 infection. In Journal of veterinary science, 22, e36. doi:10.4142/jvs.2021.22.e36. https://pubmed.ncbi.nlm.nih.gov/34056877/
3. Dias, Andreia, Santos, Diana, Coelho, Teresa, Sousa, Alda, Lemos, Carolina. 2019. C1QA and C1QC modify age-at-onset in familial amyloid polyneuropathy patients. In Annals of clinical and translational neurology, 6, 748-754. doi:10.1002/acn3.748. https://pubmed.ncbi.nlm.nih.gov/31019999/
4. Cui, Hong-Kai, Tang, Chao-Jie, Gao, Yu, Zhang, Jian, Li, Yong-Dong. 2023. An integrative analysis of single-cell and bulk transcriptome and bidirectional mendelian randomization analysis identified C1Q as a novel stimulated risk gene for Atherosclerosis. In Frontiers in immunology, 14, 1289223. doi:10.3389/fimmu.2023.1289223. https://pubmed.ncbi.nlm.nih.gov/38179058/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen