C57BL/6JCya-C3em1flox/Cya
Common Name:
C3-flox
Product ID:
S-CKO-01478
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
C3-flox
Strain ID
CKOCMP-12266-C3-B6J-VA
Gene Name
Product ID
S-CKO-01478
Gene Alias
ASP; HSE-MSF; Plp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-C3em1flox/Cya mice (Catalog S-CKO-01478) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024988
NCBI RefSeq
NM_009778
Target Region
Exon 2~17
Size of Effective Region
~6.5 kb
Detailed Document
Overview of Gene Research
C3, also known as Complement 3, is the central effector molecule of the complement system [1]. It is crucial for the innate immune system, playing a key role in the detection and clearance of potential pathogens by interacting with other complement proteins [6]. C3 is at the nexus of all complement activation pathways, and its activation fragments and receptors trigger a complex network of downstream effects [4].
In C3 glomerulopathy (C3G), an uncontrolled overactivation of the alternative complement pathway occurs, leading to abnormal C3 deposition in the glomeruli and progression of renal damage [2,3,5]. In C3 glomerulonephritis (C3 GN), a subtype of C3G, abnormal activation of the alternative pathway of the complement system results in predominant C3 deposition in glomeruli [8]. Additionally, in gastric cancer, C3+ cancer-associated fibroblasts promote tumor growth, therapeutic resistance, epithelial-mesenchymal transition, and immunosuppressive effects via activation of the NF-κB signaling pathway [7].
In conclusion, C3 is essential for the normal function of the complement system in the innate immune response. Studies on diseases like C3G, C3 GN, and gastric cancer have highlighted the significance of C3 in disease pathogenesis. Understanding C3's role through these disease models provides insights into potential therapeutic strategies for complement-mediated diseases.
References:
1. Zarantonello, Alessandra, Revel, Margot, Grunenwald, Anne, Roumenina, Lubka T. 2022. C3-dependent effector functions of complement. In Immunological reviews, 313, 120-138. doi:10.1111/imr.13147. https://pubmed.ncbi.nlm.nih.gov/36271889/
2. Schena, Francesco Paolo, Esposito, Pasquale, Rossini, Michele. 2020. A Narrative Review on C3 Glomerulopathy: A Rare Renal Disease. In International journal of molecular sciences, 21, . doi:10.3390/ijms21020525. https://pubmed.ncbi.nlm.nih.gov/31947692/
3. Riedl, Magdalena, Thorner, Paul, Licht, Christoph. 2016. C3 Glomerulopathy. In Pediatric nephrology (Berlin, Germany), 32, 43-57. doi:. https://pubmed.ncbi.nlm.nih.gov/27056062/
4. Kolev, Martin, Barbour, Tara, Baver, Scott, Francois, Cedric, Deschatelets, Pascal. 2022. With complements: C3 inhibition in the clinic. In Immunological reviews, 313, 358-375. doi:10.1111/imr.13138. https://pubmed.ncbi.nlm.nih.gov/36161656/
5. Meuleman, Marie-Sophie, Grunenwald, Anne, Chauvet, Sophie. 2022. Complement C3-targeted therapy in C3 glomerulopathy, a prototype of complement-mediated kidney diseases. In Seminars in immunology, 60, 101634. doi:10.1016/j.smim.2022.101634. https://pubmed.ncbi.nlm.nih.gov/35817659/
6. Delanghe, Joris R, Speeckaert, Reinhart, Speeckaert, Marijn M. . Complement C3 and its polymorphism: biological and clinical consequences. In Pathology, 46, 1-10. doi:10.1097/PAT.0000000000000042. https://pubmed.ncbi.nlm.nih.gov/24300728/
7. Zhao, Zhenxiong, Xiong, Si, Gao, Jianpeng, Guo, Ergang, Huang, Yakai. 2024. C3+ cancer-associated fibroblasts promote tumor growth and therapeutic resistance in gastric cancer via activation of the NF-κB signaling pathway. In Journal of translational medicine, 22, 1130. doi:10.1186/s12967-024-05939-5. https://pubmed.ncbi.nlm.nih.gov/39707456/
8. Yin, Guang, Cheng, Zhen, Zeng, Cai-Hong, Liu, Zhi-Hong. . C3 glomerulonephritis in multiple myeloma: A case report and literature review. In Medicine, 95, e4843. doi:10.1097/MD.0000000000004843. https://pubmed.ncbi.nlm.nih.gov/27631242/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen