C57BL/6JCya-C5ar1em1flox/Cya
Common Name:
C5ar1-flox
Product ID:
S-CKO-01481
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
C5ar1-flox
Strain ID
CKOCMP-12273-C5ar1-B6J-VA
Gene Name
Product ID
S-CKO-01481
Gene Alias
C5aR; C5r1; Cd88; D7Msu1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-C5ar1em1flox/Cya mice (Catalog S-CKO-01481) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000168818
NCBI RefSeq
NM_001173550
Target Region
Exon 2
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
C5ar1, also known as complement 5a receptor 1, is a key receptor in the complement system. It binds to complement component C5a, a pro-inflammatory anaphylatoxin, and is involved in various inflammatory and immune-related pathways. The C5a-C5aR1 axis plays a crucial role in multiple biological processes, including the regulation of immune cell functions, inflammation, and tissue repair, and is associated with many diseases [3].
In various disease models, C5ar1 has been shown to have significant impacts. In a NASH mouse model, C5aR1 deletion reduced inflammation and fibrosis, with transcriptional profiling revealing enrichment of pathways like Toll-like receptor signaling [1]. In lupus nephritis mouse models, knocking down C5aR1 suppressed the C5a-C5aR1 axis-controlled phosphorylation of Drp1S616 and mitochondrial fission in podocytes, alleviating podocyte injury [2]. In a COVID-19 mouse model, genetic and pharmacological inhibition of C5aR1 signaling ameliorated lung immunopathology, indicating its role in COVID-19 pathophysiology [4].
In conclusion, C5ar1 is a vital receptor in the complement system, with its activation driving inflammation and tissue damage in multiple disease conditions. Studies using gene-knockout or pharmacological inhibition in mouse models have clearly demonstrated its role in diseases such as NASH, lupus nephritis, and COVID-19, suggesting that targeting C5aR1 could be a potential therapeutic strategy for these diseases.
References:
1. Jiang, Keqing, Lu, Shibang, Li, Dongxiao, He, Songqing, Zhong, Fudi. 2023. Blockade of C5aR1 alleviates liver inflammation and fibrosis in a mouse model of NASH by regulating TLR4 signaling and macrophage polarization. In Journal of gastroenterology, 58, 894-907. doi:10.1007/s00535-023-02002-w. https://pubmed.ncbi.nlm.nih.gov/37227481/
2. Ye, Baokui, Chen, Binfeng, Guo, Chaohuan, Yang, Niansheng, Zhang, Hui. 2024. C5a-C5aR1 axis controls mitochondrial fission to promote podocyte injury in lupus nephritis. In Molecular therapy : the journal of the American Society of Gene Therapy, 32, 1540-1560. doi:10.1016/j.ymthe.2024.03.003. https://pubmed.ncbi.nlm.nih.gov/38449312/
3. Ruocco, Anna, Sirico, Anna, Novelli, Rubina, Aramini, Andrea, Amendola, Pier Giorgio. 2022. The role of C5a-C5aR1 axis in bone pathophysiology: A mini-review. In Frontiers in cell and developmental biology, 10, 957800. doi:10.3389/fcell.2022.957800. https://pubmed.ncbi.nlm.nih.gov/36003145/
4. Silva, Bruna M, Gomes, Giovanni F, Veras, Flavio P, Proost, Paul, Cunha, Thiago M. 2023. C5aR1 signaling triggers lung immunopathology in COVID-19 through neutrophil extracellular traps. In The Journal of clinical investigation, 133, . doi:10.1172/JCI163105. https://pubmed.ncbi.nlm.nih.gov/37104043/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen